Figure legends
Figure 1. I-E genotype annual distribution of 34 unusual Rotavirus group A strains isolated from children aged ≤16 years hospitalized with acute gastroenteritis during 2007-2021.
Abbreviation: EUD= unidentified E genotype.
Figure 2. Phylogenetic tree of VP6 gene of unusual Rotavirus group A strains (n=34) circulating in Greece between 2007-2021. Reference strains are indicated by a colored circle. The tree was constructed using the Maximum Likelihood method and Tamura 3-parameter model.19 A discrete Gamma distribution was used to model evolutionary rate differences among sites. Bootstrap values (1000 replicates) above 70% are shown. The scale bar indicates the branch length for 5% nucleotide differences. Evolutionary analysis was conducted with MEGA 11 software.20
Figure 3 . Multiple sequence alignment of the NSP4 proteins (n=28) of unusual human Rotavirus group A strains. E1, E2 and E3 strains were compared to Wa (AF093199.1), DS-1 (EF672582.1), and AU-1 (D89873.1), respectively.
Abbreviations: H1= Hydrophobic domain 1, H2= Hydrophobic domain 2, H3= Hydrophobic domain 3, GS1= Glycosylation site 1 and GS2= Glycosylation site 2.
Figure 4. Phylogenetic tree of NSP4 gene of unusual RVA strains circulating in Greece between 2007-2021. Reference strains are indicated by a colored circle. The tree was constructed using the Maximum Likelihood method and Tamura 3-parameter model.19 A discrete Gamma distribution was used to model evolutionary rate differences among sites. Bootstrap values (1000 replicates) above 70% are shown. The scale bar indicates the branch length for 5% nucleotide differences. Evolutionary analysis was conducted with MEGA 11 software.20