Figure legends
Figure 1. I-E genotype annual distribution of 34 unusual
Rotavirus group A strains isolated from children aged ≤16 years
hospitalized with acute gastroenteritis during 2007-2021.
Abbreviation: EUD= unidentified E genotype.
Figure 2. Phylogenetic tree of VP6 gene of unusual Rotavirus
group A strains (n=34) circulating in Greece between 2007-2021.
Reference strains are indicated by a colored circle. The tree was
constructed using the Maximum Likelihood method and Tamura 3-parameter
model.19 A discrete Gamma distribution was used to
model evolutionary rate differences among sites. Bootstrap values (1000
replicates) above 70% are shown. The scale bar indicates the branch
length for 5% nucleotide differences. Evolutionary analysis was
conducted with MEGA 11 software.20
Figure 3 . Multiple sequence alignment of the NSP4 proteins
(n=28) of unusual human Rotavirus group A strains. E1, E2 and E3 strains
were compared to Wa (AF093199.1), DS-1 (EF672582.1), and AU-1
(D89873.1), respectively.
Abbreviations: H1= Hydrophobic domain 1, H2= Hydrophobic domain 2, H3=
Hydrophobic domain 3, GS1= Glycosylation site 1 and GS2= Glycosylation
site 2.
Figure 4. Phylogenetic tree of NSP4 gene of unusual RVA strains
circulating in Greece between 2007-2021. Reference strains are indicated
by a colored circle. The tree was constructed using the Maximum
Likelihood method and Tamura 3-parameter model.19 A
discrete Gamma distribution was used to model evolutionary rate
differences among sites. Bootstrap values (1000 replicates) above 70%
are shown. The scale bar indicates the branch length for 5% nucleotide
differences. Evolutionary analysis was conducted with MEGA 11
software.20