To identify which fungi isolated from the soil had the potential for
extracellular colorant production, a visual evaluation of the colorants
produced by the fungi in the culture medium was carried out. The fungus
strains were transferred to tubes containing Czapeck-Dox-agar and
incubated at room temperature for 14 days. After colony growth, the
colorants present in the culture medium and soluble in ethyl acetate
were observed. This solvent was transferred to tubes and shaken for 30
seconds in a tube shaker and maintained at room temperature for 30 min.
Afterwards, the solvent was removed and the presence of color in the
solvent was evaluated [15]. The eight fungal strains that showed the
most intense colorants were submitted to a submerged bioprocess.