5. Conclusion
In summary, DEHTP showed a regular oscillation pattern in the multigenerational reproductive toxicities, with stimulation in F1 and F3 generations and inhibition in F2 and F4 ones. The influences of DEHTP on reproduction and lifespan showed trade-off relationships. Further analysis demonstrated the involvement of lipid metabolism and neural regulation underlying the multi-generational effects. HCA results pointed out the oscillatory connection between 5-HT and other neural regulation in F1 and F3 and between 5-HT and other indicators in F2 and F4. The overall oscillatory effects of DEHTP over generations need further transcriptomic and epigenomic analysis to explore the underlying mechanisms.