2.2. Nematode preparation
Escherichia coli (E. coli ) OP50 was cultured in lysogeny broth (LB) medium at 37 ºC and served as the food for subsequent nematode culture. Caenorhabditis elegans (C. elegans , wild-type N2) nematodes were cultured at 20 ºC on nematode growth medium (NGM) agar. Gravid hermaphrodite nematodes were lyzed by fresh Clorox solution (1% NaClO and 0.5 mol/L NaOH), and the eggs were resistant to such lysis and therefore were age-synchronized for subsequent experiments (Li et al., 2019).