cell wall |
cell wall |
cell wall |
cell wall |
cell wall |
cell
wall |
3D STORM
(dual-color imaging)
|
Membrane N-acetylglucosamineprotein A
enterotoxin B
|
nile red
WGA (AF647)
Indirect immunofluorescence staining (AF647)
Indirect immunofluorescence staining (AF647)
|
fixed
|
Staphylococcus aureus
|
10.1186/s12915-022-01472-3
|
dSTORM |
peptidoglycan |
azido-D-Ala-D-Ala probe linked to AF647 |
fixed |
Streptococcus pneumoniae |
10.1016/j.xpro.2021.101006 |
dSTORM/
3D-SIM
|
penicillin-binding proteins
glycan strands
|
CephCy5
azide N-acetylmuramic acid (AzNAM)
|
fixed
|
Staphylococcus aureus
|
10.1021/acschembio.2c00741
|
STED/SIM
|
peptidoglycan
|
Fluorescein/OregonGreen488/ TAMARA-labelled d-amino acids
|
fixed
|
Staphylococcus aureus
Streptococcus mutans
Bacillus subtilis
Escherichia coli
Zymomonas mobilis
|
10.1099/mic.0.000996
|
3D-SIM |
Z-ring (SepF and FtsZ) |
indirect immunofluorescence staining
(AF555 and AF488) |
fixed |
Methanobrevibacter smithii |
10.1038/s41467-021-23099-8 |
3D-SIM |
bactofilin (CcmA) |
HaloTag (JF549) |
fixed |
Helicobacter pylori |
10.7554/eLife.80111 |
SIM |
teixobactin |
fluorescent teixobactin analogues (fluorescein, Cy3
and Cy5) |
fixed |
Bacillus subtilis
|
10.1039/d2sc01388f |
DNA |
DNA |
DNA |
DNA |
DNA |
DNA |
PALM
(SMT)
|
DNA methyltransferase (DnmA)
|
PAmCherry
|
live
|
Bacillus subtilis
|
10.1128/mbio.03185-22
|
PALM
(SMT)
|
translesion synthesis polymerases (Pol IV)
single-stranded DNA-binding protein (SSB)
|
PAmCherry, mYPet
|
live
|
Escherichia coli
|
10.1073/pnas.2208875119
|
PALM (imaging/
SMT)
PAINT
|
two-component system SsrB, SsrA
membranes
|
PAmCherry
Nile red
|
fixed/live
|
Salmonella enterica
|
10.7554/eLife.45311
|
PALM (imaging/
SMT)
3D-SIM
|
RNA polymerase
Nucleoid
|
PAmCherry
HU-mCherry
|
fixed/live
|
Escherichia coli
|
10.1073/pnas.1507592112
|
PALM (imaging/
SMT)
|
DNA polymerase I
ligase
|
PamCherry
|
fixed/live
|
Escherichia coli
|
10.1073/pnas.1301804110
|
SIM
|
extracellular polysaccharides
eDNA
|
fluorescein isothiocyanate-labeled concanavalin A
propidium iodide
|
fixed
|
Bacillus subtilis
Brevundimonas diminuta
|
10.1007/s11356-022-21645-5
|
secretion systems |
secretion systems |
secretion systems |
secretion systems |
secretion systems |
secretion systems |
2D/3D-MINFLUX/
STORM/
STED
|
T3SS translocon
(YopD)
T3SS basal body
(YscD)
|
ALFA-Tag/NbALFA based labelling (AbberiorSTAR635P, AF647)
Halo-Tag (AF647)
|
fixed
|
Yersinia enterocolitica
|
10.1088/2050-6120/aca880
|
PALM (SMT) |
T3SS basal body (YscD) |
PAmCherry |
live |
Yersinia
enerocolitica |
10.1038/s41467-021-21863-4 |
PALM (imaging/
SMT)
|
SecYEG translocon
ATPase (SecA)
|
Ypet/mEos3.2
|
fixed/
live
|
Escherichia coli
|
10.1038/s41598-021-81081-2
|
2D/3D-PALM
(dual-color imaging)
|
T3SS
PrgH, SpaO, OrgA, OrgB, InvC, InvI, InvA
SipD
|
mEos3.2
indirect immunofluorescence staining (flag-tag, AF647)
|
fixed
|
Salmonella enterica
|
10.1073/pnas.1705823114
|
dSTORM
(dual-color imaging/
SMT)
|
flagellar basal body complex (FliN)
T1SS ATPase subunit (SiiF)
T1SS Secretin (SiiC)
T3SS export aparatus (SpaS)
T3SS ATPase (InvC)
|
Halo (TMR, Atto6)
SNAP (TMR-Star)
|
fixed/
live
|
Salmonella enterica
|
10.1038/srep31601
|
2D/3D-STED (dual-color imaging)/
SIM
|
T3SS translocon
(YopB, YopD, LcrV)
T3SS basal body
YscD
|
indirect immunofluoescence staining (AbberiorSTARRed and AF594)
GFP
|
fixed
|
Yersinia enterocolitica
|
10.1371/journal.ppat.1007527
|
2D/3D-STED
|
T3SS translocon
(YopD)
|
ALFA-Tag/NbALFA based labelling (AF647, AbberiorSTAR635P)
|
fixed
|
Yersinia enterocolitica
|
10.1371/journal.ppat.1010251
|
2D/3D-SIM
|
T6SS proteins (TssB,TssA)
Type six secretion dynamic localization protein A
(TslA)
|
sfGFP/mCherry2/mNeonGreen
indirect immunofluorescence staining (AF594)
|
fixed/live
|
Acinetobacter spp.
Burkholderia thailandensis
|
10.15252/embj.2021108595
|
SIM
|
T9SS components (GldL and GldM)
Adhesin (SprB)
|
indirect immunofluoescence staining (AF488, AF561)
|
fixed
|
Flavobacterium johnsoniae
|
10.1371/journal.pbio.3001443
|
SIM |
T4SS protein (VirB6a) |
sfGFP |
fixed |
Agrobacterium
tumefaciens |
10.1074/jbc.RA118.002751 |
adhesins, virulence factors |
adhesins, virulence
factors |
adhesins, virulence factors |
adhesins,
virulence factors |
adhesins, virulence factors |
adhesins, virulence factors |
DNA-PAINT |
fibronectin binding proteins (FnBPA and FnBPB) |
Fn
conjugated to a DNA-PAINT docking sequence, Cy3B-labeled complementary
imager strands |
fixed |
Staphylococcus aureus |
10.1021/acsnano.2c00630 |
PAINT/
dSTORM
(dual-color imaging)
|
listeriolysin S
membrane
|
Direct immunofluoescence staining (HA-tag, AF647)
Nile red
|
fixed
|
Listeria monocytogenes
|
10.1073/pnas.2108155118
|
PALM, dSTORM;
dSTORM/PALM (dual-color imaging)
|
F-type ATPase
(F1-like-X0)
protease (MIP0582)
|
mEos3.2
indirect immunofluoescence staining (HA-tag, AF 647)
|
fixed
|
Mycoplasma spp.
|
10.1128/spectrum.00645-22
|
SIM |
hemolysin A (HlyA) |
eGFP/ Indirect immunofluoescence staining
(Cy3 or AbberiorStar635P) |
fixed |
Escherichia coli |
10.1128/AEM.01896-21 |