at cpDNA loci
For cpDNA, we used the SNPs dataset obtained for the 94 individuals (90M. fragrans , 4 M. argentea ) to describe the geographic
distribution of cpDNA genetic diversity. We estimated the haplotype
number (H ), number of segregating sites (S), and nucleotide
diversity (π) using DnaSP v.6.12.03 . To analyze the genetic variation
among groups and populations, we assigned the populations on each island
to two different regions: Ambon and Banda were considered as a Southern
Moluccas group and Ternate, Tidore and Bacan as a Northern Moluccas
group. We used a molecular variance analysis (AMOVA) approach in
ARLEQUIN v.3.5 assessing significant differences with 1,000
permutations.