2.3 | Collection of soil samples
Soil samples were collected 15 days after the end of the grazing treatment in July. Soil samples were collected using the S-type sampling method in each experimental plot of each grazing intensity treatment in the test area, including CK. The above-ground vegetation and litter cover were first removed and the topsoil of the surface 1 cm was removed. The soil punch was inserted into the soil, and t The 0-10㎝ thick surface soil was taken as soil samples using the soil drill, with 6 replicates in each treatment plot. Soil samples were taken 6 times for each grazing intensity treatment as 6 replicates. After the collection was completed, the collected soil samples were sealed in sterile plastic bags and placed in a 4°C environment for temporary storage at low temperature, and then transported back to the laboratory for follow-up analysis. The soil holes caused by sample collection, should be filled select soil from natural grazing pasture by those soil outside the sample plot should be filled to minimize human disturbance.