Genomic run-on
Genomic run-on (GRO) was performed as described in (García-Martínez et
al., 2004) and as modified in (Oliete-Calvo et al., 2018). Briefly, GRO
detects by macroarray hybridization, genome-wide, active elongating RNA
polymerase (RNA pol) II, whose density per gene is taken as a
measurement of its synthesis rate (SR). At the same time, the protocol
allows the mRNA amounts (RA) for all the genes to be measured. mRNA
half-lives (HLs) are calculated as RA/SR by assuming steady-state
conditions for the transcriptome. The total SR for a given yeast strain
was calculated as the sum of individual gene SRs. GRO datasets are
available at the Gene Expression Omnibus (GEO) with accession numbers:
GSE29519 and GSE198240 (reviewer access code: mbyjqgschrcfhib ).