Figure 4
Angiotensin II-induced increase in brain immune cell infiltration is blood pressure-dependent. A: Representative flow cytometry dot plots showing gating strategy for total leukocytes (CD45+ high) from the brains of mice infused with vehicle, angiotensin II, vehicle + hydralazine and angiotensin II + hydralazine. The effect of angiotensin infusion and co-treatment with hydralazine on B:CD45+ leukocytes, C: CD3+ T cells, D: CD4+ T cells,E: CD11b+ myeloid cells, F: Ly6G+ neutrophils,G: Ly6C+low monocytes, H:Ly6C+high monocytes, I: F4/80+ macrophages and J: CD19+ B cells in the brain (n=7-8). All data are mean ± S.E.M. *P<0.05. Two-way ANOVA with Tukey’s test.