3.2. The presence of ESBL and AmpC in CFEC bacteria
ESBL was detected in 28.6% (n=35) of 122 E. coli. Also, AmpC was
not detected in any of the isolates (0 %). The genes responsible for
ESBL and AmpC resistance were investigated with the PCR only in E.
coli which are phenotypic ESBL-positive (Table 3). In this respect,bla SHV gene was not detected in any of the 35
isolates, and the bla CTX-M-1 gene was detected at
the highest rate (68.5%). It was found that 3 of the ESBL isolates
contained 4 of the screened resistance genes. In another 4 isolates, 3
of the genes were screened and 2 different genes were detected in 21
isolates. While only one of the genes was detected in 6 isolates, none
of the tested genes were detected in one isolate.
One or more of the other genes were detected in all isolates that
contained the bla OXA-10. Similarly, no isolates
that contained only one bla TEM were detected,
while all isolates containing the blaTEM gene as well asthe bla TEM were detected as one or more of the
CTX-Ms or bla OXA-10 genes. Although phenotypic
negative results, bla CIT andbla MOX genes were also investigated in
ESBL-positive isolates and were not detected in any isolates. All
results are presented in Table 4. The coding of ESBL detectedE.coli is the same as the numbering in CFEC, and CFEC-ESBL is
abbreviated by adding ESBL in front of this abbreviation.