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\begin{document}

\title{MICROMORPHOLOGICAL AND PHYTOCHEMICAL PROFILING OF DELPHINIUM SUAVE HUTH.
FROM HINDUKUSH RANGE, LOWER DIR KHYBER PAKHTUNKHWA PAKISTAN}



\author[1]{Imran Ahmad}%
\author[1]{Dr.Ghulam Dastagir}%
\affil[1]{University of Peshawar Department of Botany}%


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  \date{May 25, 2024}


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\begin{abstract}
This is a very first attempt to study various parameters of a medicinal
plant,~\emph{Delphinium suave} Huth. The plant is erect, geophytic,
herbaceous, with tuberous root, trifid in a palmatipartite, strigose
cuneate leaf and white spurred zygomorphic flower. The root was
isodiametric phellem with single non-glandular trichomes. The stem
revealed single-layered cuticle, multiseriate epidermis, cortex, pith
ray and uniserate bowed non-glandular trichomes. The leaf was
amphistomatic, showed tapering trichomes, prismatic crystals and
ranunculaceous stomata with circumference 144.66-182.67 \selectlanguage{greek}µ\selectlanguage{english}m. Pollen
grains in Light Microscopy (LM), were prolate, spheroidal
trizonocolpate, isopolar, radiosymmetric, scabrate, elliptic and monads.
Scanning Electron Microscope (SEM) pollen surface was scabrate, monad,
size varied from 18.06 \selectlanguage{greek}µ\selectlanguage{english}m-16.67\selectlanguage{greek}µ\selectlanguage{english}m, colpus to inaperturate, tricolpate,
ornamented, echinus, isopolar, isodiametric and circular. SEM roots
showed sclerenchymatic tissues, stellate, glandular, non-glandular
trichomes and crystals. The stem showed scalariform, pitted vessels,
warty protuberances, unicellular, silicified, non-glandular trichomes.
Leaves powder revealed, simple, unicellular, tapered headed, uniseriate,
sessile, capitate, unbranched glandular, non-glandular, trichomes with
crystals. Capitate, stellate, circular, unicellular, branchy trichomes
were observed for the first time through SEM. Powder drug study of root,
stem leaves through LM also revealed different tissues. Preliminary
phytochemical revealed alkaloids, anthocyanins, anthraquinones,
coumarins, flavones, mucilages, saponins, steroids, terpenoids, volatile
oils and proteins. GC/MS showed 36 compounds in roots 33 in stem while
40 in leaves. Fluorescence analysis of roots, stem and leaves showed
variation in colour when treated with chemicals. This study will assist
pharmacognostic exploration, authentication from adulterants/allied
species for consistent quality, resulting in safe use, preservation and
efficacy.%
\end{abstract}%



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