Analysis of Differentially Expressed Proteins in Lymph Fluids
Related to Lymphatic Metastasis in Breast Cancer Rabbit Model Guided by
Contrast-Enhanced Ultrasound
Jiachao Xu1,2+, Xin Zhang1,2+,
Guangfei Yang2, Wei Sun1,2, Wen
Wang2*, Chengrong Mi2*
1Ningxia Medical University, Yinchuan, China
2General Hospital of Ningxia Medical University,
Yinchuan, China
[Grant Project] National Natural Science Foundation of China
(82260351)
Abstract: Objective: To identify differentially expressed proteins in
the lymph fluid of rabbits with breast cancer lymphatic metastasis and
normal rabbits, and to analyze and verify using proteomics technology.
Materials and Methods: Rabbits with breast cancer lymph node metastasis
and healthy rabbits were selected. Lymph fluids were precisely extracted
under the guidance of percutaneous contrast-enhanced ultrasound (CEUS).
Label-free quantitative proteomics (LFQ) was used to detect and compare
differences, and differential protein expression was obtained.
Bioinformatics analysis was performed using KEGG and GO analysis
software, selecting the most significantly differentially expressed
proteins. Finally, parallel reaction monitoring technology (PRM) was
applied for validation.
Results: A total of 547 significantly differentially expressed proteins
were found in this study, including 371 upregulated proteins and 176
downregulated proteins. They were mainly involved in various cellular
and metabolic pathways, including upregulated proteins such as BLVRA,
IDH2, and downregulated proteins such as PDXK. The upregulated proteins
PDIA3, PRKAR1A, and ABCC4 participated in immune regulation, endocrine
regulation, and anti-tumor drug resistance regulation, respectively.
Conclusion: Compared with normal rabbits, rabbits with breast cancer
metastasis have various differentially expressed proteins in their lymph
fluid. These proteins are involved in the pathophysiological processes
of tumor development and metastasis, and can be used as predictive
indicators for breast cancer metastasis and as new targets for
treatment.
【Key words】 Breast cancer; Lymph node metastasis;
Contrast-enhanced ultrasonography;Proteomics; Differentially expressed
protein