3.7 Emerging SRN techniques (MINFLUX, MINSTED, RASTMIN, RESI)
Several SRN techniques have emerged recently that can achieve resolution on the scale of the size of fluorescence emitters (Figures 2 and 3, Supporting Table 1). These techniques bear the potential to yield the resolution of a few nanometres or even several Angstroms in 3D and bridge the gap between optical microscopy, electron microscopy, and structural biology techniques. Emerging SRN techniques are challenging to implement and require subnanometer probes and subnanometer sample position stabilisation. However, they can yield combined structural and dynamic characterisation of interactions between signalling proteins and can even accurately identify conformational changes within individual proteins with nanometre resolution in living cells. The most promising techniques include MINFLUX, MINSTED, RASTMIN, RESI, and photoswitching fingerprint analysis.