3.7 Emerging SRN techniques (MINFLUX, MINSTED, RASTMIN, RESI)
Several SRN techniques have emerged recently that can achieve resolution
on the scale of the size of fluorescence emitters (Figures 2 and 3,
Supporting Table 1). These techniques bear the potential to yield the
resolution of a few nanometres or even several Angstroms in 3D and
bridge the gap between optical microscopy, electron microscopy, and
structural biology techniques. Emerging SRN techniques are challenging
to implement and require subnanometer probes and subnanometer sample
position stabilisation. However, they can yield combined structural and
dynamic characterisation of interactions between signalling proteins and
can even accurately identify conformational changes within individual
proteins with nanometre resolution in living cells. The most promising
techniques include MINFLUX, MINSTED, RASTMIN, RESI, and photoswitching
fingerprint analysis.