8.3 Light sheet fluorescence microscopy
LSFM or selective plane illumination microscopy (SPIM) is a powerful
tool for imaging long-term processes in large samples with minimal
photodamage to the sample (Figure 2o) (Voie et al., 1993). LSFM uses a
light sheet of laser light to illuminate a thin sample section
perpendicular to the observation direction. Therefore, LSFM minimises
the duration of illumination for individual parts of the sample, reduces
phototoxicity, and enables long-term observations with good axial
resolution. LSFM has been used for imaging downstream signalling from
activated adrenergic receptors in acute brain slices (Pham et al.,
2020).