8.3 Light sheet fluorescence microscopy
LSFM or selective plane illumination microscopy (SPIM) is a powerful tool for imaging long-term processes in large samples with minimal photodamage to the sample (Figure 2o) (Voie et al., 1993). LSFM uses a light sheet of laser light to illuminate a thin sample section perpendicular to the observation direction. Therefore, LSFM minimises the duration of illumination for individual parts of the sample, reduces phototoxicity, and enables long-term observations with good axial resolution. LSFM has been used for imaging downstream signalling from activated adrenergic receptors in acute brain slices (Pham et al., 2020).