Plant Materials and generation of CSGGCT2;1 overexpression
lines
Camelina sativa (L.) Crantz cultivar ’Sunseon’ was used as the
wildtype plant. CsGGCT2;1 overexpression lines were generated by
inoculating wild-type plants with Agrobacterium via vacuum
infiltration (Lu and Kang 2008). Agrobacterium tumefaciens strain
EHA105A with modified pCAMBIA1300 plasmid containingCaMV35Sp::CsGGCT2;1::Nos-t construct was used for transformation
(Figure 1a). After transformation, homozygous lines were generated using
DsRED as a selection marker (Figure 1b). The gene expression level was
confirmed by using isolated RNA from 3-week-old plants, synthesizing
cDNA, and using quantitative real-time PCR (qRT- PCR) (Figure 1c).
T3 homozygous lines were used for all analyses.