Plant Materials and generation of CSGGCT2;1 overexpression lines
Camelina sativa (L.) Crantz cultivar ’Sunseon’ was used as the wildtype plant. CsGGCT2;1 overexpression lines were generated by inoculating wild-type plants with Agrobacterium via vacuum infiltration (Lu and Kang 2008). Agrobacterium tumefaciens strain EHA105A with modified pCAMBIA1300 plasmid containingCaMV35Sp::CsGGCT2;1::Nos-t construct was used for transformation (Figure 1a). After transformation, homozygous lines were generated using DsRED as a selection marker (Figure 1b). The gene expression level was confirmed by using isolated RNA from 3-week-old plants, synthesizing cDNA, and using quantitative real-time PCR (qRT- PCR) (Figure 1c). T3 homozygous lines were used for all analyses.