Analysis of GSH and γ-EC levels
The levels of GSH and γ-EC were analyzed via HPLC in WT and twoCsGGCT2;1 OE lines (#102 and #103) exposed to MS control and AsIII treatments. There were no significant differences in GSH levels between WT and OE lines under MS control or in response to AsIII treatment (Figure 7a). However, under AsIII treatment, overall GSH levels increased relative to MS control (Figure 7a). For γ-EC analysis,CsGGCT2;1 OE lines had significantly (50-70%) lower γ-EC levels than WT under MS control, but under AsIII treatment, there were no significant differences between WT and OE lines (Figure 7b). As with GSH, overall γ-EC levels increased in the plants grown on AsIII stress relative to MS control (Figure 7b).