Additional files
Additional file 1: Figure S1. CRISPR/Cpf1 mediated homologous recombination. C. glutamicum was transformed with the pJYS3_Amp_DT_[Lp-Knr-Lt] vector. The vector was incorporated into the genomic DNA after the first cross-linking in the promoter region of the ldhA gene. The vector accessories and theldhA gene are arranged in tandem. The selection marker gene (Knr) on the vector part (A) and ldhA gene (B) was concomitantly detected.
Additional file 2: Table S1. Primers design to construct CRISPR-cpf1 gene editing vector.
Table 1. Hydrolysis rate (%) of softwoods and hardwoods depending on pretreatment methods.