Fig. 3. Synergistic effect of cellulase with xylanase and β-glucosidase. (A) HPAC-pretreated pine with cellulase cocktail solution alone and (B) supplement with xylanases (Xyl) and β-glucosidases (Bgls) were conducted in 1 mL citrate buffer. The incorporation of auxiliary enzymes remarkably increased the hydrolysis rate of the 2% substrate and decreased the dose of cellulase required for optimal hydrolysis (5–25 FPU). The structural recalcitrance of cellulose fiber did not substantially affect the enzymatic hydrolysis of HPAC-pretreated pine.