Materials.
Citrulline (C7629), 2,3-Butanedione monoxime (B0753), Antipyrine
(A5882), Iron (lll) Chloride (157740), A1AT, Bovine serum albumin
(A9418), and PNPP (S0942) were purchased from Sigma-Aldrich, MO.
Antibodies including anti-NE (MAB9167, Novus Biologicals, CO), anti-A1AT
(Thermo Fisher Scientific, MA), anti- peptidylarginine deiminase 4
(PAD4, ab128086, Abcam, MA), anti-MPO (14569P, Cell-signaling MA),
anti-thyroid transcription factor-1 (TTF-1, SPT24, Biocare Medical, CA),
anti-elastin (MA5-41583, Thermo Fisher Scientific, MA) and anti-histone
H3 pan antibodies (07-690, Upstate, NY) were used in this study. Soluble
elastin (Elastin Products Company, MO). Secondary antibodies for Western
blot were purchased from Li-Cor Biosciences, NE, and anti-Rabbit IgG-AP
(Southern Biotech, AL). Reagents including EnVision FLEX Target
Retrieval Solution High pH (Dako/Agilent, CA) and low pH IHC Antigen
Retrieval Solution (Invitrogen, MA), Biocare Decloaking Chamber
(Biocare Medical, CA) and Tris-Buffered Saline (TBS) (Dako/Agilent, CA)
were used in immunohistochemistry.