Materials.
Citrulline (C7629), 2,3-Butanedione monoxime (B0753), Antipyrine (A5882), Iron (lll) Chloride (157740), A1AT, Bovine serum albumin (A9418), and PNPP (S0942) were purchased from Sigma-Aldrich, MO. Antibodies including anti-NE (MAB9167, Novus Biologicals, CO), anti-A1AT (Thermo Fisher Scientific, MA), anti- peptidylarginine deiminase 4 (PAD4, ab128086, Abcam, MA), anti-MPO (14569P, Cell-signaling MA), anti-thyroid transcription factor-1 (TTF-1, SPT24, Biocare Medical, CA), anti-elastin (MA5-41583, Thermo Fisher Scientific, MA) and anti-histone H3 pan antibodies (07-690, Upstate, NY) were used in this study. Soluble elastin (Elastin Products Company, MO). Secondary antibodies for Western blot were purchased from Li-Cor Biosciences, NE, and anti-Rabbit IgG-AP (Southern Biotech, AL). Reagents including EnVision FLEX Target Retrieval Solution High pH (Dako/Agilent, CA) and low pH IHC Antigen Retrieval Solution (Invitrogen, MA), Biocare Decloaking Chamber (Biocare Medical, CA) and Tris-Buffered Saline (TBS) (Dako/Agilent, CA) were used in immunohistochemistry.