2.1 Determination of cAMP content in spores
The standard cAMP concentration (0.3125–0 pmol/50 μL) and its corresponding absorbance were linearly correlated. Based on the measured data, the standard curve is shown in Figure 1, and the following regression equation is obtained: Y = −0.2214X + 0.1949. In the formula, Y represents the measured absorbance value, X represents the standard cAMP concentration (pmol/50 μL), and R2 = 0.9368.
The cAMP content of the measured sample was obtained from the standard curve. Finally, cAMP concentrations in dormant and non-dormant chlamydospores of SDH035 and DH055 isolates were obtained, and the results are shown in Figures 2A and 2B. As shown in the figure, the content of cAMP in resting spores of both strains was significantly higher than that in non-resting spores (p <0.05).