2.2 Determination of protein content in spores
After measurement, the concentration curve of standard protein is shown
in Figure 3. In the regression equation y=1.362x + 0.9675, R2=0.9555, Y
represents the measured average absorbance value, and X represents the
standard protein concentration.
Based on the absorbance values,
the standard curve was substituted to obtain the protein concentration
of the measured samples. The average value of total protein in each
107 spores was finally obtained (Figures 4A and B). As
shown in the figure, the protein content of dormant spore in DH055 was
significantly higher than that of non-dormant spore
(p <0.05), and the protein content of dormant spore in
SDH035 was slightly higher than that of non-dormant spore, but the
difference was not significant (p >0.05).