2.2 Determination of protein content in spores
After measurement, the concentration curve of standard protein is shown in Figure 3. In the regression equation y=1.362x + 0.9675, R2=0.9555, Y represents the measured average absorbance value, and X represents the standard protein concentration.
Based on the absorbance values, the standard curve was substituted to obtain the protein concentration of the measured samples. The average value of total protein in each 107 spores was finally obtained (Figures 4A and B). As shown in the figure, the protein content of dormant spore in DH055 was significantly higher than that of non-dormant spore (p <0.05), and the protein content of dormant spore in SDH035 was slightly higher than that of non-dormant spore, but the difference was not significant (p >0.05).