Genetic sampling and library preparation
Sample site locations were chosen to maximize sampling coverage across
the breeding and nonbreeding ranges of the American Redstart. We used
genetic samples from a total of 330 individuals: 182 individuals from 16
locations across the breeding range and 148 individuals from 15
locations in the nonbreeding range (Table S1). Sample collection
occurred between 1993 and 2022 and consisted of either blood from
brachial venipuncture or feathers. We extracted DNA from blood samples
using the standard protocol for Qiagen DNEasy Blood and Tissue Kits and
we modified the protocol to maximize DNA yield from feathers. Whole
genome sequencing libraries were prepared following modifications of
Illumina’s Nextera Library Preparation protocol . Pooled libraries were
sequenced on eight HiSeq 4000 lanes at Novogene Corporation Inc with a
target sequencing depth of 2X per individual.