Table 1 : Comparison between the reported literature on TMAO
detection.
Here we report a method for assaying the enzyme trimethylamine
monooxygenase in which the TMAO formed is directly quantified. The
presence of trimethylamine interferes with the previously reported
methods for TMAO detection (Chang et al. , 2021a). We overcome
this issue by reacting residual trimethylamine using a derivatizing
reagent iodoacetonitrile (IACN), that does not interfere with the assay
(Hefni et al., 2021). Thus, our detection method becomes TMAO-specific
in the enzymatic assay.