4.7 Interference Study
4.7.1 Interference due to trimethylamine : Three different reactions were set up with the spiked concentration of TMAO. The concentrations ranged from 20 µM to 80 µM of TMAO while keeping the trimethylamine constant at 50 µM. The high concentration of trimethylamine was chosen because it is the substrate of our enzyme. The controls did not have IACN to remove the trimethylamine.
4.7.2 Metal ion: The method developed was checked for metal ion interference; 50 µM of metal ion solutions were used in the reaction mixture with one mM TMAO. As mentioned in section 2.5, the reaction was performed and studied for metal ion interference.