Result
Results from isolated mitochondria, cells with different
permeabilization percentages, and cells with damaged mitochondria
demonstrated the ability of capturing ATP signals specifically from the
mitochondria. Breast cancer PBMCs exhibited a significant increase in
glutamic acid- and malic acid-induced mitochondrial ATP synthesis
capacity yet a significant decrease in mitochondrial DNA copy number
(mtDNA CN) compared to healthy PBMCs. Furthermore, breast cancer PBMCs
mostly showed negative correlation between mtDNA CN and parameters from
MitoRAISE but healthy individuals showed positive correlation.