2.2 Permeabilization of cellular plasma membrane
Cells or PBMCs were treated with 5–10 nM plasma membrane permeabilizer (PMP) and incubated at room temperature for 10 min. Membrane permeabilization percentage was measured with the percentage of trypan blue stained cells using a Countess automated cell counter (Thermofisher Scientific, Waltham, MA, USA). The acceptable permeabilization rate was 90% or higher in the PMP-treated cells.