Figure 2. MitoRAISE validation in cell lines
A) Total ATP contents of A549 cells at increasing cell count and A549 rho-0 cells found through commercial kit compared with the ATP contents measured through MitoRAISE assay. B) GM-induced and S-induced ATP synthesis capacity of A549 cells at different cell count, A549 rho-0 cells, and A549 cells treated with CCCP or FCCP C~F) Correlation between mitochondrial parameters in cell lines.
CCCP: 2-[2-(3-Chlorophenyl)hydrazinylyidene]propanedinitrile, FCCP: carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone, GM: glutamic acid and malic acid, S: succinate