2.2 Permeabilization of cellular plasma membrane
Cells or PBMCs were treated with 5–10 nM plasma membrane permeabilizer
(PMP) and incubated at room temperature for 10 min. Membrane
permeabilization percentage was measured with the percentage of trypan
blue stained cells using a Countess automated cell counter (Thermofisher
Scientific, Waltham, MA, USA). The acceptable permeabilization rate was
90% or higher in the PMP-treated cells.