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\begin{document}

\title{Upper-critical solution temperature (UCST) polymer functionalized
nanomedicine for controlled drug release and hypoxia alleviation in
tumor therapy}



\author[1]{Xiaoya  Niu}%
\author[2]{Yi  Fu}%
\author[2]{Lei  Feng}%
\author[2]{Maodi  Xie}%
\author[2]{Bei  Li}%
\author[2]{Lin  Que}%
\author[1]{Zhen You}%
\affil[1]{West China Hospital of Sichuan University}%
\affil[2]{Affiliation not available}%


\vspace{-1em}



  
  \date{May 24, 2024}


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\selectlanguage{english}
\begin{abstract}
Recently, nanomedicine have been successfully applied in the cancer
therapy. However, how to precisely control the drug release from
nanomedicine in tumor tissue and overcome the hypoxic microenvironment
of tumor tissue is still an important challenge in the development of
nanomedicine. In this work, a new type of drug-loaded nanoparticles
P(AAm-co-AN)-AuNRs@CeO2-DOX (PA-DOX) was prepared by combining
high-efficiency photothermal reagents, critical up-conversion
temperature polymer layer and anti-cancer drug doxorubicin (DOX) for the
treatment of liver cancer. In this system, CeO2 can decompose hydrogen
peroxide to H2O2 and O2 alleviate the anaerobic microenvironment of
liver cancer cells. As a photothermal reagent, AuNRs@CeO2 can convert
near-infrared light into heat energy to achieve local heat to kill
cancer cells and ablate solid tumors. In addition, the elevated
temperature would enable the polymer layer to undergo a phase transition
to release more DOX to achieve a controlled release mechanism, which
will open up a new horizon for clinical cancer treatment%
\end{abstract}%



\sloppy


Dear editor:

We would like to submit the enclosed manuscript, entitled
``\textbf{Upper-critical solution temperature (UCST) polymer
functionalized nanomedicine for controlled drug release and hypoxia
alleviation in tumor therapy}

'' for your consideration. No conflict of interest exits in the
submission of this manuscript, and manuscript is approved by all authors
for publication. I would like to declare on behalf of my co-authors that
the work described was original research that has not been published
previously, and not under consideration for publication elsewhere, in
whole or in part. I outline briefly below the significant findings
reported in this manuscript and reasons why this manuscript deserves
serious consideration for publication as a Original research (article)
for ``\textbf{Biotechnology Journal} ''.

This manuscript displayed a new type of drug-loaded nanoparticles
P(AAm-co-AN)-AuNRs@CeO\textsubscript{2}-DOX (PA-DOX) for the treatment
of liver tumors. Among them, CeO\textsubscript{2} can decompose hydrogen
peroxide to alleviate the anaerobic microenvironment of liver cancer
cells. As a photothermal reagent, AuNRs@CeO\textsubscript{2} can convert
near-infrared light into heat energy to achieve local heating to kill
cancer cells and ablate solid tumors. What's more, P(AAm-co-AN) was used
as the ucst polymer layer. Under low temperature conditions, the polymer
layer protects the DOX. When the laser irradiation achieves a local
temperature rise, the polymer layer undergoes a phase transition to
release more drugs to achieve a controlled release mechanism, which will
open up new horizons for clinical treatment. Animal experiments have
proved the possibility of nano drug delivery system and good tumor
treatment effect. I hope this paper is suitable for
``\textbf{Biotechnology Journal} ''.

We deeply appreciate your consideration of our manuscript, and we look
forward to receiving comments from the reviewers. If you have any
queries, please don't hesitate to contact me at the address below. Thank
you and best regards. Yours sincerely,

Zhen You

\textbf{Hosted file}

\verb`manuscript.docx`  available at \url{https://authorea.com/users/622949/articles/645854-upper-critical-solution-temperature-ucst-polymer-functionalized-nanomedicine-for-controlled-drug-release-and-hypoxia-alleviation-in-tumor-therapy}

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