RESULTS
This study involved 32 rats divided into Control, MTX, MTX+RAN, and RAN+MTX groups. The experiment was terminated in the Control and MTX+RAN groups without mortality, while 1 rat from the MTX group and 2 rats from the RAN+MTX group died during the experiment. The same number of rats was added to these groups to maintain equal numbers. Before the end of the experiment, the weight of rats and liver was measured and found to be comparable among the groups (p >0.05) (Figure 1).
The study analyzed serum biochemical findings, tissue biochemistry, and histopathological results. The results showed a statistically significant increase in serum AST, ALT, ALP, and LDH levels in the MTX group compared to the Control group. However, the MTX+RAN and RAN+MTX groups resulted in significant improvement compared to the MTX group. The biochemical results were presented in Figure 2.
MDA values were significantly increased in the MTX group, while CAT value decreased compared to the Control group. In the MTX+RAN group, the SOD value showed a significant decrease compared to the Control group, whereas SOD and the MDA value showed a significant decrease compared to the MTX group. When the RAN+MTX group was examined, MDA value significantly decreased compared to MTX and MTX+RAN groups. However, no significant change was observed for tGSH levels among the groups. The results were presented in Figure 3.