Reduced Glutathione Measurement
GSH levels were measured using the method reported by Akerboom and Sies
(Akerboom and Sies, 1981). This measurement method involves
spectrophotometric measurement at 412 nm of the increase in
5-tio-2-nitrobenzoate (TNB) formation as a result of the reaction of
DTNB, NADPH, and glutathione reductase. The absorbance change was
monitored by UV-vis pectrophotometry at 412 nm for 15 min. The results
were presented in nmol/mg protein.