Histopathological Results
Microscopic livers of rats belonging to the control group were observed with normal morphological structure. Histopathological results showed a significant increase in mononuclear inflammation, vascular congestion, ductal proliferation, and vacuolization consistent with hepatocyte damage in the MTX group compared to the Control group (p <0.001). The RAN+MTX and MTX+RAN groups showed a significant reduction in vascular congestion and ductal proliferation compared to the MTX-treated group alone (p <0.001). There was no significant difference in Roening grades observed when the RAN+MTX and MTX+RAN groups were compared with the MTX group (Figure 4). The administration of RAN was found to be promising in preventing and alleviating histopathological damage. Overall, the study suggests that RAN administration can prevent hepatocyte damage caused by MTX.