Homogenization of tissues
Liver samples weighing 0.1 g were collected in plastic tubes and
supplemented with 1 mL of pH 7.4 PBS buffer. The tissue samples were
homogenized using a homogenizer under ice isolation until complete
disintegration. The resulting homogenates were sonicated four times at
30-second intervals using a sonificiper under ice insulation.
Subsequently, the homogenates were centrifuged for 15 minutes at 14,000
rpm and +4°C using a cooled centrifuge. Finally, the obtained
supernatants were stored at +4°C until further analyses.