Determination of catalase activity
The activity of the CAT enzyme was analyzed using Aebi method (Aebi,
1974), which is based on the absorbance of H2O2 at 240 nm. Initially,
the absorbance was adjusted to 0.7-0.9 by adding concentrated H2O2 to
the Na-K-phosphate buffer solution (pH: 7). Subsequently, 1 mL of the
prepared mixture was placed in a quartz bath, and the final supernatant
was added. After gentle stirring, the absorbance of H2O2 (ε= 0.0396
cm2/μmol) was monitored using the Shimadzu 1601-UV visible
spectrophotometer for 60 sec at 240 nm. The results were expressed as
U/mg protein.