Homogenization of tissues
Liver samples weighing 0.1 g were collected in plastic tubes and supplemented with 1 mL of pH 7.4 PBS buffer. The tissue samples were homogenized using a homogenizer under ice isolation until complete disintegration. The resulting homogenates were sonicated four times at 30-second intervals using a sonificiper under ice insulation. Subsequently, the homogenates were centrifuged for 15 minutes at 14,000 rpm and +4°C using a cooled centrifuge. Finally, the obtained supernatants were stored at +4°C until further analyses.