Malondialdehyde Measurement
Measurement of MDA was determined using the method previously reported by Placer et al (Placer et al., 1966). This method is based on the measurement of the pink-colored product formed at 532 nm as a result of the reaction between MDA, the breakdown product of peroxidized lipids, and thiobarbituric acid (TBA). The standard used in this method was 1,1,3,3-tetraethoxypropane. The MDA content of the samples was expressed as nmol/g protein.