Prediction of binding affinity of peptides to TAP

Peptides must be transported to the endoplasmic reticulum before they can be presented to CD4+ cells by MHC-I. TAP protein carries out the transport process, so the TAP affinities of the eight amino acid length peptides were evaluated and shown in Table-3.

Allergenicity prediction

The identical peptides allergenicity predicted in AllerTOP v2.0 bioinformatic tool and shown in Table-3. Identical epitopes causing autoimmunity should not necessarily be allergic.

Toxicity prediction

The identical peptides must be non-toxic to the human body. For prediction of toxicity, the ToxinPred tool used for toxicity prediction results is shown in Table-3.
Table-3
Evaluation of the transport efficiency through to TAP protein complex involved in antigen presentation, allergenicity, and toxicity of 8mer sequences posessing molecular mimicry.