ISAC ImmunoCAP
The Immuno-solid phase allergen chip (ISAC) ImmunoCAP testing was carried out according to the manufacturers instructions. This enables testing for specific IgE against 112 allergen components in a multiplex assay (supplementary data). Results were measured with a biochip scanner (Capital bio Lux scanner) and evaluated using specialized software (MIA Array software) as per manufacturers instructions. The results were reported in ISAC Standardized Units (ISU-E) and interpretation was done according to international guidelines [17] as shown below.
<0.3 ISU-E: Undetectable
0.3 to 0.9 ISU-E: Low
1 to 14.9 ISU-E: Moderate/High
>15 ISU-E: Very High