FlowCam setup details for analysis with the 20x objective FlowCam setup details for analysis with the 20x objective
Flow cell sizes and types used, and objectives used for each flow cell 50x300 μm flow cell (FOV), 20x objective
Calibration factor 0.3380
Image acquisition mode Auto-image
Flow rate 0.03 mL min-1
Example of full context settings for 20180816_0356_001_NSP
Run: Mode: AutoImage Priming Method: machine prime Flow Rate: 0.030 ml/min Recalibrations: 1 Stop Reason: Sample Volume Processed Sample Volume Aspirated: 1.0000 ml Sample Volume Processed: 0.9990 ml Fluid Volume Imaged: 0.3388 ml Efficiency: 33.9% Particle Count: 1239 Images: Total: 38201 Used: 1219 Percentage Used: 3.19% Particles Per Used Image: 1.02 Particles Per Image: 0.03 Particles Per Image: 0.03 Frame Rate: 19.57 fps Intensity Mean: 177.09 Intensity Min: 174.78 Intensity Max: 177.72 Date/Time: Start: 2019-08-22 10:48:26 End: 2019-08-22 11:21:52 Sampling Time: 00:32:31 Environment: Software: VisualSpreadsheet 4.15.1 Magnification: 20X Calibration Factor: 0.3380 GumStix Firmware: 23 Calibration Factor: 0.3380 SerialNo: 10446 Number of Processors: 8 Pump: C80 Syringe Syringe Size: 0.50 ml Capture Parameters: Distance To Neighbor=30 Threshold Dark=15.0000 Threshold Light=18.0000 Close Holes=0 Capture Dark or Light Pixels=2