3.1 Enzymatic nanoreactor synthesis and functionalization
The complementary electrostatic charge between the negatively charged GOx enzyme surface and the positively charged inner face of the BMV capsid drive the enzyme encapsulation (Fig. 1a). The isoelectric point of GOx was determined by electrophoretic light scattering using the Z potential at different pH and showed to be 4.6 (SI 1). The self-assembly process was modulated by ionic strength (0.75 M) at pH 5.1. The GOx enzyme was successfully encapsulated inside VLPs from BMV by the self-assembly synthesis method (Fig. 1b).