2.1 Materials
Type VII glucose oxidase from Aspergillus niger , human serum albumin (HSA, 96%), dextrose (99.5%), hydrogen peroxide (30 wt%), guaiacol (99%), L-glutathione reduced (98%), and all other general reagents, precursors and solvents were purchased from Sigma-Aldrich (St. Louis, MO). Salts used for buffer solutions were purchased at Fermont (Monterrey, MX). TEM analysis was performed using copper grids (400-mesh) coated with formvar/carbon support film (Ted-Pella, USA). All chemicals were of the highest grade commercially available. Cancer cell lines were acquired at ATTC (USA).