3.1 Enzymatic nanoreactor synthesis and
functionalization
The complementary electrostatic charge between the negatively charged
GOx enzyme surface and the positively charged inner face of the BMV
capsid drive the enzyme encapsulation (Fig. 1a). The isoelectric point
of GOx was determined by electrophoretic light scattering using the Z
potential at different pH and showed to be 4.6 (SI 1). The self-assembly
process was modulated by ionic strength (0.75 M) at pH 5.1. The GOx
enzyme was successfully encapsulated inside VLPs from BMV by the
self-assembly synthesis method (Fig. 1b).