Figure 6 . Cytotoxicity of unfunctionalized VLP-GOx and
functionalized VLP-GOx@HSA nanoreactors on 293T kidney cell line.
The cytotoxicity was also assayed in 293T healthy embryonic kidney human
cells (Fig. 6). The results were expressed as both GOx protein content
and nanoreactor GOx activity. The LD50 values were 0.45
µg GOx/mL for VLP-GOx and around 0.79 µg GOx/mL for VLP-GOx@HSA, and no
metabolic activity was detected at 1 µg GOx/mL for VLP-GOx preparation
(Fig. 6a). The VLP-GOx nanoreactors on 293T kidney cells showed an
LD50 at around 15 mU of GOx activity per mL of cell
culture, and no metabolic activity at around 30 mU/mL (Fig. 6b). On the
other hand, the VLP-GOx@HSA nanoreactors showed an LD50around 8 mU/mL (Fig. 6b).