Statistical analysis
A two-sided Student‘s t-test with unequal variances (Welch‘s t-test) was
done in GraphPad Prism version 5.01 (GraphPad Software, San Diego, CA,
USA). Other statistical analyses were done in R vers. 4.2.1 (R Core
Team, 2022). The Alamar Blue and corresponding OD600measurements shown in Fig. 1 and Fig. S2 were modelled with splines
using the bamlss package version 1.1-8 (Umlauf et al. ,
2021). The modelling was done separately for each strain and condition
(Alamar Blue fluorescence signal and OD600 signal). The
model description is bamlss(value ~ s(Time_min),
family=”gaussian”). The growth curves in Fig. 4, 7, and 9 were done
using the growthcurver package and ggplot2 (Wickham, 2016;
Sprouffske and Wagner, 2016). All figures were plotted using R/ggplot2
in RStudio, except in Fig. 8B using GraphPad.