Fig. 2. Characterization and quantification of ΔybeX and Keio wild-type (WT) colony sizes at 37°C and 42°C. (A ) Representative LB agar plates with colonies of WT and ΔybeXstrains. As indicated on the panel‘s top, the cells were plated from LB or MOPS minimal media and the agar plates were incubated at 37°C overnight. (B-C ) Density plots of the distribution of quantified colony radiuses of ΔybeX and isogenic WT strains at 37°C and 42°C. The colonies were quantified from three independent experiments as of the plates in panel A. (D ) Colony counts for WT and ΔybeX strains grown in LB or MOPS minimal media as in panel A. Values of four biological replicates with three technical replicates each are shown.