Construction of pET-based YbeY overexpression plasmid
The pET-ybeY overexpression plasmid was constructed using the CPEC
(Circular Polymerase Extension Cloning) method (Quan and Tian, 2011).
The primers were designed to amplify the pET-Orange plasmid backbone and
the coding region of the ybeY gene from the E. coli genome
(sequences are detailed in Table S3). Phusion™ HF DNA Polymerase (Thermo
Scientific, #F530L) was used following the manufacturer protocol.