Statistical analysis
A two-sided Student‘s t-test with unequal variances (Welch‘s t-test) was done in GraphPad Prism version 5.01 (GraphPad Software, San Diego, CA, USA). Other statistical analyses were done in R vers. 4.2.1 (R Core Team, 2022). The Alamar Blue and corresponding OD600measurements shown in Fig. 1 and Fig. S2 were modelled with splines using the bamlss package version 1.1-8 (Umlauf et al. , 2021). The modelling was done separately for each strain and condition (Alamar Blue fluorescence signal and OD600 signal). The model description is bamlss(value ~ s(Time_min), family=”gaussian”). The growth curves in Fig. 4, 7, and 9 were done using the growthcurver package and ggplot2 (Wickham, 2016; Sprouffske and Wagner, 2016). All figures were plotted using R/ggplot2 in RStudio, except in Fig. 8B using GraphPad.