Fig. 3. ΔybeX cells exhibit severe sensitivity to sub-MIC concentrations of cold-shock-inducing, ribosome-binding antibiotics.(A ) The wild-type (WT) and the ΔybeX cells were grown overnight in LB liquid medium, serially diluted, and spotted on LB agar plates supplemented with sub-MIC concentrations of indicated antibiotics (No AB). The plates were incubated at 37°C overnight. (B ) Representative images are given from a dot spot assay with strains described in Fig. 1. pYbeX denotes the YbeX-expressing TransBac library plasmid.