Bacterial Spot Assay
Bacterial cell cultures were diluted to final OD600 = 0.125, the first dilution (10-1), and then 10x serial dilutions were applied. 5µL of each dilution were spotted on LB agar plates with or without antibiotics (No AB) supplementation. The sub-inhibitory concentrations of the antibiotics were as follows; 2.5-4 µg/mL chloramphenicol, 0.5-1 µg/mL tetracycline, 20-40 µg/mL erythromycin, 50 µg/mL clindamycin, 50-100 µg/mL fusidic acid, 10-20 µg/mL mupirocin, 0.5 µg/mL tobramycin or amikacin, 2.5 µg/mL rifampicin, 2.5 µg/mL streptomycin. The plates were imaged using an Epson Expression 1680-pro scanner.