Bacterial Spot Assay
Bacterial cell cultures were diluted to final OD600 =
0.125, the first dilution (10-1), and then 10x serial
dilutions were applied. 5µL of each dilution were spotted on LB agar
plates with or without antibiotics (No AB) supplementation. The
sub-inhibitory concentrations of the antibiotics were as follows; 2.5-4
µg/mL chloramphenicol, 0.5-1 µg/mL tetracycline, 20-40 µg/mL
erythromycin, 50 µg/mL clindamycin, 50-100 µg/mL fusidic acid, 10-20
µg/mL mupirocin, 0.5 µg/mL tobramycin or amikacin, 2.5 µg/mL rifampicin,
2.5 µg/mL streptomycin. The plates were imaged using an Epson Expression
1680-pro scanner.