Growth monitoring in the 96-well plate reader
C ells were diluted in the appropriate growth media to OD600 = 0.55, and 10 µL of the diluted cells were transferred into 100 µL of growth media in a 96-well plate. The 96-well plate edges were filled with distilled water. The remaining 60 wells were used to monitor the growth. At least one column was always set as a sterility control. Alamar Blue reagent (BioRad, #BUF012B) was used per the manufacturer’s protocol (excitation 545 nm, emission 590 nm). BioTek Synergy Mx or H1 microplate readers were used.