Growth monitoring in the 96-well plate reader
C ells were diluted in the appropriate growth media to
OD600 = 0.55, and 10 µL of the diluted cells were
transferred into 100 µL of growth media in a 96-well plate. The 96-well
plate edges were filled with distilled water. The remaining 60 wells
were used to monitor the growth. At least one column was always set as a
sterility control. Alamar Blue reagent (BioRad, #BUF012B) was used per
the manufacturer’s protocol (excitation 545 nm, emission 590 nm). BioTek
Synergy Mx or H1 microplate readers were used.