Figure 4: Intracellular acidification is linked to killing of
CoNS but not non-toxic S. aureus.
After phagocytosis and lysostaphin/gentamicin treatment for 1 h (t0)
THP-1-CWT cells were further incubated for 3 h or 24 h in medium
containing gentamicin to kill extracellular/escaped bacteria. At
indicated time points, the cells were lysed and bacterial CFU determined
(A ). Membrane integrity of the THP-1, CWT cells were assessed
by LDH-assay (B ).
The data represent at least three biological replicates. Significance
was determined by one-way analysis of variance with Tukey‘s multiple
comparison post-test.