Figure 3: Role of ROS detoxifying enzymes and regulatory system
for bacterial survival.
After phagocytosis and lysostaphin/gentamicin treatment for 1 h (t0)
THP-1-CWT cells (A, B, C, D ) or hMDM (E, F ) were
further incubated for 3 h or 24 h in medium containing gentamicin to
kill extracellular/escaped bacteria. At indicated time points, the cells
were lysed and bacterial CFU determined (A, C, E ). Membrane
integrity of the THP-1-CWT or hMDM cells were assessed by LDH-assay
(B, D, F ). The data represent at least three biological
replicates Significance was determined by one-way analysis of variance
with Tukey‘s multiple comparison post-test.