Strain construction
All derivatives of the USA300 agr/sae mutants were obtained by
transduction of specific mutations from the Nebraska mutant library (Fey
et al., 2013) into strain USA300 agr/sae using Φ11 lysates. All
mutants were confirmed by PCR using oligonucleotides spanning the
transposon insertion sites. For expression of sodM inS. epidermidis , sodM was amplified using
oligonucleotides 5’‑CCAGTGAATTCGAGCTCAAATTATATTAAGTTATATTATTTTGCTGCTTGGT-3
and 5’‑ATGATGGTACCGTTAACAACACACCCGAAATTAATTATT-3. The construct was
cloned into the AHT-inducible vector pCG248. The generated plasmid
pCG817 was transduced into S. epidermidis 1457 using S.
aureus PS187 Δsau ΔhsdR and the phage Φ187.Transductants were verified with 5’-CAAAATTATACATGTCAACGA-3’ and
5’‑AAGCAGCTCTAATGCGCTGT-3’