Live-Imaging
For Live-cell Microscopy cells were infected with bacteria constitutively expressing mCherry. THP1-CWT cells were seeded in a 4 Compartment Cell Culture Dish (Greiner) and infection was performed like already described. Live-Imaging was started at t0 with a LSM800 microscope (Zeiss) using a 40 x objective with immersion oil. The yellow fluorescent protein (YFP) channel received an excitation wavelength of 488 nm, an emission wavelength of 509 nm and a detection wavelength of 490-575 nm. For detection of mCherry, an excitation wavelength of 587 nm, an emission wavelength of 610 nm and a detection wavelength of 565-700 nm were used.