Hyper-production of porcine contagious pleuropneumonia subunit vaccine proteins in Escherichia coli by developing a bicistronic T7 expression system
Manman Sun1,2#, Alex Xiong Gao3#, An Li1,4,5#, Rodrigo Ledesma-Amaro2, Zhonghu Bai1,4,5, Yankun Yang1,4,5, Xiuxia Liu1,4,5*
Co-first author
* Correspondence:liuxiuxia@jiangnan.edu.cn
1 National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214112, China
2 Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK
3 Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, China
4 Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
5 Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, 214122, China