2.8 Challenge experiment with A. pleuropneumoniaeserotype 7
The A. pleuropneumoniae serotype 7 strain used in this study was
provided by Tecon Biology Co., Ltd and was cultured in BHI medium
supplemented with 10 mg/L β -nicotinamide adenine dinucleotide at
37 °C.[26] For the challenge experiment, forty
mice were divided into four groups: Three experimental groups were
immunized with 20 μg purified Oml1, Oml7, or ApxII, respectively. The
control group was immunized with PBS. After 14 days of immunization, all
mice were intraperitoneally injected with 0.5 mL of A.
pleuropneumoniae serotype 7 (6×108 CFU). The survival
of mice was counted in the following 7 days.