Fig. 2 Characterization of effects of different fore‑cistron sequences on the performance of bicistronic T7 expression system
C (control) represents the monocistronic expression vector pET28-EGFP. The abscissa represents the BCD vector containing the corresponding fore-cistron. (A) EGFP expression level of the BCD expression vectors. (B) The relative transcriptional level and relative translation efficiency of EGFP in bicistronic T7 expression system.