Hyper-production of porcine contagious pleuropneumonia subunit
vaccine proteins in Escherichia coli by developing a bicistronic
T7 expression system
Manman Sun1,2#, Alex Xiong Gao3#,
An Li1,4,5#, Rodrigo
Ledesma-Amaro2, Zhonghu Bai1,4,5,
Yankun Yang1,4,5, Xiuxia Liu1,4,5*
#Co-first author
* Correspondence:liuxiuxia@jiangnan.edu.cn
1 National Engineering Research Center of Cereal
Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi
214112, China
2 Department of Bioengineering and Imperial College
Centre for Synthetic Biology, Imperial College London, London SW7 2AZ,
UK
3 Division of Life Science, The Hong Kong University
of Science and Technology, Hong Kong, China
4 Key Laboratory of Industrial Biotechnology, Ministry
of Education, School of Biotechnology, Jiangnan University, Wuxi 214122,
China
5 Jiangsu Provincial Research Center for Bioactive
Product Processing Technology, Jiangnan University, Wuxi, 214122, China