Fig. 2 Characterization of effects of different fore‑cistron
sequences on the performance of bicistronic T7 expression system
C (control) represents the monocistronic expression vector pET28-EGFP.
The abscissa represents the BCD vector containing the corresponding
fore-cistron. (A) EGFP expression level of the BCD expression vectors.
(B) The relative transcriptional level and relative translation
efficiency of EGFP in bicistronic T7 expression system.