Cell proliferation assay
The proliferation of human OS cells was evaluated by the CellTiter 96
AQueous One Solution cell proliferation assay. human OS cells were
plated in 96-well culture plates (3 × 103 per well).
After 24 h of incubation, the cells were transfected with 30 pmol of
target gene (sh-circ_0001722 or miR-204-5p or negative control) for 24,
48, 72, and 96 h. Then 20 μl Cell Titer 96 Aqueous One Solution
(Promega, USA) were added and cells incubated for another 1 h.
Absorbance was read at 492 nm.