Xenograft nude mouse model
Six-week-old male BALB/C nude mice (Vital River Laboratory Animal
Technology, Beijing, CHN) were maintained under specific pathogen-free
conditions with a 12h light/dark cycle. All animal experiments were
performed in accordance with the guide lines for the Care and Use of
Laboratory Animals of Zhengzhou University. MG63 cells stably
transfected with sh-circ_0001722 lentivirus or control lentivirus were
subcutaneously injected into the right upper back of the nude mice
(5×106 cells per mouse). Four weeks later, the mice
were sacrificed and tumor tissues were collected for examination of the
parameters of interest.