Figure 3. circ_0001722 binds to miR-204-5p to suppress its expression in human OS cells. a: Computational algorithms predicts complementary sequences of the circ_0001722 and miR-204-5p binding sequence. b: Compared with miR-NC, miR-204-5p mimic can significantly reduce the luciferase activity of pmirGLO-Wt-circ clone in HEK293T cells. However, neither miR-NC nor miR-204-5p mimic can affect the luciferase activity of pmirGLO-Mt-circ clone. c: Ago2 RIP assay shows that Ago2 can significantly enrich circ_0001722 and miR-204-5p. d: Downregulation of circ_0001722 can promote miR-204-5p expression in both human OS cell lines. e: The expression of miR-204-5p was negatively associated with circ_0001722 in human OS tissues. NC: negative control. Wt: wild type. Mt: mutant type. P <0.01.