Abstract
Osteosarcoma (OS) is the most prevalent primary fatal bone neoplasm in
adolescents and children owing to limited therapeutic methods. Circular
RNAs (circRNAs) are identified as vital regulators in a variety of
cancers. However, the role of circRNAs in OS are still unclear. In our
study, we evaluate the differentially expressed circRNAs in 3 paired OS
and corresponding adjacent nontumor tissue samples by circRNA microarray
assay, finding a novel circRNA, circ_001722, significantly upregulated
in OS tissues and cells. Then we show that downregulation of
circ_0001722 can suppress proliferation and invasion of human OS cellsin vitro and in vivo . Computational algorithms predict
miR-204-5p can bind with circ_0001722 and RUNX2 mRNA 3’UTR, which is
verified by Dual-luciferase assay and RNA immunoprecipitation assay.
Further functional experiments show that circ_0001722 competitively
binds to miR-204-5p and prevents it to decrease the level of RUNX2,
which upregulates proliferation and invasion of human OS cells. In
summary, our research firstly show that circ_001722 promotes the
progression of OS via miR-204-5p/RUNX2 axis.
Keywords: Osteosarcoma; circRNA; miR-204-5p; Runx2;
Proliferation; Invasion