Figure 5. sh-circ_0001722 suppresses proliferation and invasion of human OS cells through miR-204-5p/RUNX2 axis. a: Computational algorithms predicts that miR-204-5p targets RUNX2 mRNA 3’UTR. b: Comparing with miR-NC, miR-204-5p can significantly reduce the luciferase activity of pmirGLO-Wt-3’UTR clone in HEK293T cells. However, neither miR-NC nor miR-204-5p can affect the luciferase activity of pmirGLO-Mt-3’UTR clone. c: Both miR-204-5p and sh-circ_0001722 can suppress RUNX2 expression in human OS cells. d: Suppression affect by downregulating circ_0001722 on cell proliferation can be rescued by exogenous RUNX2. e: Suppression affect by downregulating circ_0001722 on cell invasion can be rescued by exogenous RUNX2. NC: negative control. Wt: wild type. Mt: mutant type.P <0.05. P <0.01.