Figure 1. Identification and validation of differential expression of circ_0001722 in OS tissues and corresponding adjacent nontumor tissues. a: CircRNA expression profiling between two groups is showed with volcano plot. The vertical lines refer to a 2.0-fold (log2 scaled) up-regulation and down-regulation, respectively. The horizontal line corresponds to a P value of 0.05 (−log10 scaled). The red points represent up regulated circRNAs with statistical significance, and green points represent down regulated ones. b: Hierarchical clustering heatmap indicates differences in circRNA expression profiling between the two groups. c: 11 target miRNAs of circ_0001722 are predicted by miRanda and TargetScan, as well as their corresponding target cancer related mRNA, estimated by miRTarBase database. Green: circ-0001722; red: miRNAs; blue: mRNAs. d: Relative levels of circ_0001722 and GAPDH in OS cell line MG63 after their RNAs are treated with or without RNase R digestion. e: The validation of back-splicing junction sites of circ_0001722 by Sanger sequencing. f: The relative expression of circ_0001722 was detected in 20 OS tissues and corresponding adjacent nontumor tissues. (b) The relative expression of circ_0001722 was detected in two OS cell lines and an immortalized human fetal osteoblastic cell line.P <0.01.