2.4. Preparation of standard solution and electrochemical measurement of glucose biosensors
Glucose standard solutions with concentrations of 1, 3, 10, 50, and 100 mM were prepared and stored in a refrigerator at 37.5°C before performing the electrochemical measurement. The RE, CE, and WE were connected to a potentiostat (WizECM-1200 premium, Wizmac, Korea), and the cyclic voltammetric (CV) and chronoamperometric (CA) measurements were performed at room temperature. CV measurements indicated the oxidation and reduction of glucose and were performed at a scan rate of 100 mV·s−1 for the potential range between −1.0 and +1.0 V. CA measurements were performed for 20 s at regular intervals of 3 s at a detection potential of +0.6V for various glucose concentrations, such as 1, 3, 10, 50, and 100 mM.