Materials
Elamipretide 60 mg was provided by Stealth BioTherapeutics (Needham, MA, USA) as 0.75 mL SC injections. Mometasone ointment 0.1% (NDC 68462-225-55) was manufactured by Glenmark Pharmaceuticals Limited and DuoDEM Extra Thin dressing (product number 187955) was manufactured by Convatec. The suppliers for tacrolimus ointment 0.1% (NDC 0168-0416), doxepin cream 5% (NDC 0378-8130-45), and diphenhydramine 25 mg tablets (NDC 50580-226-51) were Fourgera Pharmaceuticals Inc., Mylan Pharmaceuticals Inc., and Johnson & Johnson Consumer Inc, respectively. Fluo-4 AM (LOT#2566212) and eBioScience cell stimulation cocktail 500x (REF 2024-04-18) containing ionmycin were supplied by Invitrogen by Thermo Fisher Scientific. DMEM high glucose medium (4.5 g/l) (LOT#2472338), 10% fetal bovine serum, non-essential amino acid solution (REF 1140-050, LOT#2301965), HEPES (1M) stock (REF 15630-080, LOT#2192897), and active Geneticin (G418) 50 mg/mL (REF 101315, LOT#2389171) were supplied by Gibco. Evans blue in saline and 100 ng/mL human stem cell factor were supplied by Sigma. MRGPRX2-transfected Chem-1 cells and their parental cell line, Chem-1 cells, were obtained through Eurofins Discovery.