Diagnosis
The diagnostic analysis is essential to confirm MPXV infection and to
rule out other infectious diseases. Samples of skin lesions are the best
clinical samples for laboratory analysis, such as swabs of vesicular
lesions, exudates, or crusts kept in cool, dry sterile tubes [57].
Viral cultures should be obtained by oropharyngeal or nasopharyngeal
swabs [57]. Medical history, clinical symptoms, and laboratory
studies help to diagnose MPXV infection. Laboratory studies include PCR,
ELISA, Western Blot, and immunohistochemistry, and according to WHO,
RT-PCR is the test of choice to identify MPXV during severe infection
[2].