Results
In Group A (n=8), where the rats were subjected to transtympanic
instillation of 25% ethanol henna extract, no vestibular dysfunction
was observed at 4 hours, 24 hours, 48 hours, 72 hours, one week, two
weeks, and three weeks post-instillation. Similar results were observed
in Group B (n=8), where the rats were subjected to transtympanic
instillation of 25% aqueous henna extract, and Group C (n=8), where the
control group received transtympanic instillation of normal saline.
Provided the scores were 0 for the baseline and all post-test parameters
(Table 1), statistical analysis could not be performed for this
research. In other words, there was no impairment of all vestibular
parameters documented post-instillation of both aqueous and ethanol
henna extract in the middle ear, indicating that Henna has no
vestibulotoxic effects after it is administered into the middle ear
mucosa.
This study used 24 Wistar Kyoto rats consisting of 8 male rats for
Groups A, B, and C, with the latter serving as a control group. The rats
were three months old when this study was performed and weighed between
300 to 400g each.