3.4. Histopathological Examinations
In the H&E sections, there was normal heart histology in the control group (Figure 3a). - In the hearts of all rats in the other groups, contraction bands with necrosis in focal areas, karyolysis characterized by the absence of nuclei in myocytes, were interpreted as focal and histopathological changes consistent with early stage myocardial infarction associated with ischemia. The SALD and SAHD groups had more common and significant signs of ischemic changes compared to the other groups. These changes were observed to be compatible with 4-12 hours of ischemia. The wavy appearance in myocytes was such that their cytoplasm contained eosinophilia and contraction band (Figure 3b and 3c).