Figure 3. a.Normal heart histology. H&E x100, b. Wavy appearance in myocytes and increased eosinophilia in SALD and SAHD groups (arrowhead). H&E x100, c. In the SALD and SAHD groups, areas consistent with the findings of ischemia accompanied by karyolysis (arrow), which are more clearly seen in the lower right and upper left part of the figure. H&E x100.
Desmin expression in groups was demonstrated in brown with 3,3’-Diaminobenzidine (DAB) chromogen by using immunohistochemistry method. When the severity and prevalence of staining with desmin antibody in the groups were evaluated; Weak, moderate and intense staining patterns were observed. It was noted that the control group showed a moderate and homogeneous staining pattern in terms of staining intensity and prevalence. This pattern was consistent with the staining pattern seen in myocytes in normal heart tissue. In SALD and SAHD group cases in which contraction bands were prominent, desmin accumulation was observed to be intense, especially in myocyte firming aggregates. In the cases of these two groups, it was noted that desmin stained weakly in terms of staining intensity and intensity in the ischemia areas seen as small foci (Figure 4b and 4c). Staining of SALD and SAHD group cases with desmin antibody was consistent with histomorphologically observed early signs of acute myocardial infraction and ischemia findings.