2.1. Materials
In this study, we used JWH-018 (CAS No: 209414-07-3; Lipomed, Switzerland) compound with molecular formula C24H23NO. It was stored at +4°C until JWH-018 was used. JWH-018 and its metabolites were acquired from Lipomed AG Switzerland. Ketamine hydrochloride (Ketasol %10; richterpharma ag, Wels, Australia) and xylazine (Xylazinbio %2; Bioveta Ivanovice na Hané, Czech Republic) were purchased from Biotek Animal Health Products, Malatya, Turkey. Primary rabbit‐polyclonal caspase‐3 antibody (Neomarker; Lab Vision Corp., Thermo Scientific, Fremont, CA, Cambridge, UK) and mouse monoclonal desmin antibody (Santa Cruz Biotechnology, CA, USA) were used.