Vesicle size
The average size of the two fractions of vesicles was analyzed using
light-scattering technology; through intensity measurements with a
Malvern ZetaSizer Nano XL machine (Malvern Instruments Ltd., Orsay,
France), as previously described by Barrajón-Catalán et al. ,
(2010). This allowed the measurement of the particles with a size range
from 1 nm to 3 µm.