Conclusion
In conclusion, we demonstrate the synergistic effect of TSA-1-C4 plus
Tc24-C4 recombinant antigen combination by the activation of peritoneal
macrophages and CD8+ T cells from naïve mice,
eliciting the Th1 immune response through pro-inflammatory-cytokine
production by in-vitro assays. Although, the bivalent recombinant
protein strategy did not show a beneficial effect when we evaluated the
cytotoxicity activity, we observed that, either TSA-1-C4 or Tc24-C4
antigens formulated with E6020-SE adjuvant induced significant cytotoxic
activity in the infected mice. This study supports the use of the
bivalent recombinant protein strategy; however, further trials will be
necessary in order to determine whether TSA-1-C4 plus Tc24-C4
administration prevents the development of tissue injuries.