Statistical analysis
All tests were run in GraphPad Prism software version 8.0.c. Data were analyzed by one-way ANOVA or Kruskal–Wallis tests for multiple groups, depending on its distribution followed by Tukey or Dunn’s post hoc test. When only two comparison groups were analysed, Student ̵́s t-test or Mann–Whitney U-test was performed depending on data distribution. Differences between groups were considered statistically significant when P -value was less than 0.05.