Supplementary figures
Supplementary Figure. 1 SDS-PAGE analysis at 12% of acrylamide/bis-acrylamide and staining with PageBlueTM Protein Staining Solution (ThermoFisher Scientific). Molecular weight marker (Spectra Multicolor Broad Range Protein Ladder from ThermoFisher Scientific), recombinant proteins TSA-1-C4 (65 kDa) and Tc24-C4 (24 kDa) are presented respectively.
Supplementary Figure. 2 Flow-cytometry gating strategy for cytotoxicity assay. The dot-plots show the macrophages cells gating based on (A) forward-scatter (FSC) and side-scatter (SSC) properties, (B) doublets exclusion, (C) identification of CFSE positive cells based on histograms of frequencies, (D) identification of CFSE high and CFSE low subpopulations.