Supplementary figures
Supplementary Figure. 1 SDS-PAGE analysis at 12% of
acrylamide/bis-acrylamide and staining with PageBlueTM Protein Staining
Solution (ThermoFisher Scientific). Molecular weight marker (Spectra
Multicolor Broad Range Protein Ladder from ThermoFisher Scientific),
recombinant proteins TSA-1-C4 (65 kDa) and Tc24-C4 (24 kDa) are
presented respectively.
Supplementary Figure. 2 Flow-cytometry gating strategy for
cytotoxicity assay. The dot-plots show the macrophages cells gating
based on (A) forward-scatter (FSC) and side-scatter (SSC) properties,
(B) doublets exclusion, (C) identification of CFSE positive cells based
on histograms of frequencies, (D) identification of CFSE high and CFSE
low subpopulations.