Conclusion
In conclusion, we demonstrate the synergistic effect of TSA-1-C4 plus Tc24-C4 recombinant antigen combination by the activation of peritoneal macrophages and CD8+ T cells from naïve mice, eliciting the Th1 immune response through pro-inflammatory-cytokine production by in-vitro assays. Although, the bivalent recombinant protein strategy did not show a beneficial effect when we evaluated the cytotoxicity activity, we observed that, either TSA-1-C4 or Tc24-C4 antigens formulated with E6020-SE adjuvant induced significant cytotoxic activity in the infected mice. This study supports the use of the bivalent recombinant protein strategy; however, further trials will be necessary in order to determine whether TSA-1-C4 plus Tc24-C4 administration prevents the development of tissue injuries.