Salmonella is a major pathogen worldwide causing acute foodborne outbreaks. Traditional identification methods, however, are time-consuming and faced complexity to detect contamination of bacteria in the food supply chain timely. We herein aimed to develop a method for rapid and robust detection of Salmonella Typhimurium in milk and chicken meat based on recombinase polymerase amplification (RPA) integrated with agarose gel electrophoresis (AGE). Three primers pairs were designed which function both in RPA as well as in polymerase chain reaction (PCR). A total number of 254 S. Typhimurium field isolates from various sources of North Eastern Region (NER) of India were evaluated using both RPA and PCR for validating the assay. The results were consistent in RPA and PCR-based detection using crude DNA obtained by a simple boiling method without any purification step. The RPA-AGE showed versatility functioning at 35⁰C to 41⁰C, and at the temperature of 37⁰C, it only took 5 min of amplification to reach the test threshold of amplicon. The established method had both a good specificity and a sensitivity of 10fg DNA per reaction of 15µL volume. It showed high sensitivity when artificially inoculated in fresh chicken samples even at 10^-9 fold dilutions containing 1.95 X 10¹ to 1.95 X 10⁴ cfu/mL. There was no cross-reactivity with the other four Salmonella serovars and seven bacterial pathogens tested. To our knowledge, this is the first report of reliable serovar specific detection of Salmonella Typhimurium by RPA using crude DNA extracted by a simple boiling method.