CIEF
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Ampholytes: pH 3–10 (1.5% v/v) in 30:70 glycerol/water (v/v)
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Anolyte: 50mM formic acid/1 mM glutamic acid (pH 2.35); catholyte: 100mM
NH3/1 mM lysine (pH 10.6); normal polarity (30 kV)
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50 mbar
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Bare fused silica
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Coaxial SL
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MeOH/water/acetic acid (50:50:1 v/v/v)
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Single quadrupole (Series 1100 MSD)
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Ribonuclease A,
a-chymotrypsinogen A type II, myoglobin, carbonic anhydrase II,
b-lactoglobulin A, trypsin inhibitor
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CIEF
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Ampholytes: pH 3–10 (1.5% v/v) and pH 9–11 (1% v/v) in 40/60 (v/v)
glycerol/water
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Anolyte: 50 mM formic acid/1 mM glutamic acid (pH 1.9); catholyte:
100 mM NH3/1 mM arginine (pH 11.3);
normal polarity (30 kV);
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50 mbar, 30 kV
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LPA
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Coaxial SL
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10 mM AmAc (pH 5.0)
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Ion trap (Esquire 3000+)
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Recombinant wild-type IFN-γ, single and deletion mutants
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CIEF
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For SA/ CA Ampholytes: pH 3−10 and pH 5−8 (1.5% v/v in total) in 10 mM
AmAc and 15% glycerol (pH 6.7);
For ADC Ampholytes: 0.75% (v/v) pH 3−10 and 8−10.5 with a ratio of
1:2
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Anolyte: 0.1% (v/v) formic acid (FA), 15% glycerol (pH 3.0);
catholyte: 10 mM AmBicarb, 15% glycerol, (pH 10.0); normal polarity
(30 kV);
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10 mbar, 20 kV
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LPA
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Nano SL
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10 mM AmAc, 10% (v/v) ACN (pH 5.0)
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Q-TOF (6545XT)
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Recombinant streptavidin (SA), carbonic anhydrase II (CA), interchain
cysteine-linked antibody−drug conjugate (ADC)
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