Native Capillary Electrophoresis-Mass Spectrometry for Protein analysis: Current Progress and Perspectives
Ann-Katrin Schwenzer1, Lena Kruse1, Kevin Jooß2, Christian Neusüß1
1 Department of Chemistry, Aalen University, Beethovenstraße 1, 73430 Aalen, Germany
2 Department of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States
Abbreviations: ACE, affinity capillary electrophoresis; ACD, antibody-drug conjugate; AmAc, ammonium acetate; AmBicarb ammonium bicarbonate; AmF ammonium formate; BGE background electrolyte; E. coli, Escherichia coli ; EPO, erythropoietin; ES, electrospray; FcRn; neonatal Fc receptor; HRN, high resolution native; HSN, high speed native; HOS higher order structure; iCIEF, imaged CIEF; IM-MS, ion mobility-mass spectrometry; IFN-γ, interferon-gamma; IPA, isopropanol; Kd, affinity constant; LIF, light-induced-fluorescence; LPA, linear polyacrylamide; MCE, microchip electrophoresis; MoCE, mobility capillary electrophoresis; msACE mobility shift affinity capillary electrophoresis, nanoSL, nanoflow sheath liquid, nMS native mass spectrometry, nCIEF, native capillary isoelectric focusing; nCZE, native capillary zone electrophoresis; nMCE, native microchip electrophoresis; PB-DS-PB, polybrene-dextran sulfate-polybrene; PVA, polyvinyl-alcohol; SL, sheath liquid; SMIL, successive multiple ionic polymer layer; TD, top-down; TDMS top-down mass spectrometry; UV, ultraviolet.