Separation mode
Separation conditions
Coating
Interface
Sheath liquid / Make-up liquid
MS instrument
Analyte
Ref.
CZE
BGE: 50 mM AmAc (pH 8.3); normal polarity (30 kV)
uncoated
Coaxial SL
10 mM AmAc (pH 6.8)/ MeOH (50:50, v/v)
TOF (Mariner)
Myoglobin
CZE
BGE: 50 mM AmAc (pH 7.4); reverse (20 kV), 0.7 psi
PVA
Coaxial SL
IPA/water (50:50, v/v) containing 14 mM AmAc
QTOF (micrOTOFQ, MAXIS H/D)
Antithrombin
CZE BGE: 50 mM AmBicarb (pH 7.4); normal polarity (30 kV) uncoated Coaxial SL, sheathless porous tip IPA/water/formic acid (49.5:49.5:1, v/v/v) TOF (6210 LC/MS), QTOF (maXis HD Ultra high resolution) Beta2-microglobulin
CZE
BGE: 40 mM AmAc (pH 7.5); normal polarity (20 kV), 1-3 psi
PAM
Sheathless porous tip
not applicable
Orbitrap (QE-EMR, QE Plus)
E. Coli ribosomal extract, standard proteins, mAb: trastuzumab
SEC-CZE
BGE: 50 mM AmAc (pH 6.9); normal polarity (15 kV), 1 psi
LPA
NanoSL
25 mM AmAc (pH 6.9)
Orbitrap (QE HF)
E. Coli
CZE BGE: 40 mM AmAc (pH 7.5); normal polarity (20 kV), 3 psi PAM Sheathless porous tip not applicable Orbitrap (QE Plus) mAb: IgG1
CZE
BGE: 40 mM AmAc (pH 6.0); reverse polarity (12 kV)
PB-DS-PB
Coaxial SL
IPA/water/acetic acid (50:50:0.1, v/v/v)
QTOF (QTOF 6540)
mAb: infliximab
CZE BGE: 25 mM AmAc (pH 6.8); normal polarity (30 kV), 0.5 psi LPA NanoSL 10 mM AmAc Orbitrap (modified QE-EMR) Ribosomal Proteins
CZE
BGE: 25 mM AmAc (pH 6.8); normal polarity (30 kV), 0.725 psi
LPA
NanoSL
10 mM AmAc (pH 6.8)
QTOF (6545XT AdvanceBio LC/QTOF)
mAb: SigmaMAb, NISTmAb
CZE BGE: 40 mM AmAc (pH 6.8); normal polarity (15 kV), 3 psi PAM Sheathless porous tip not applicable Orbitrap (QE-EMR, UHMR, and Eclipse) Standard proteins, GroEL
CZE
BGE: 40 mM AmAc (pH 6.8); normal polarity (18 kV), 2.2-15 psi
PAM
Sheathless porous tip
not applicable
Orbitrap (QE-EMR) Synthetic and endogenous nucleosomes
MCE (CZE)
BGE: ZipChip native Antibody kit); normal polarity (500 V/cm)
ZipChip HRN chip (native antibody kit)
ZipChip
ZipChip native Antibody kit
Orbitrap (QE Plus with extended mass Biopharma Option)
mAb: IgG4 and IgG1
MCE (CZE)
BGE: ZipChip native Antibody kit, 4% DMSO; normal polarity (500 V/cm)
ZipChip HRN chip (native antibody kit)
ZipChip
ZipChip native Antibody kit
Orbitrap (QE Plus with extended mass Biopharma Option)
mAb: rituximab, trastuzumab and bevacizumab
MCE (CZE)
BGE: ZipChip native Antibody kit, 4% DMSO; normal polarity (500 V/cm)
ZipChip HRN chip (native antibody kit)
ZipChip
ZipChip native Antibody kit
Orbitrap (QE)
mAb: IgG1
MCE (CZE) BGE: ZipChip protocol ”Boosting Sensitivity for Intact Antibody Charge Variant Analysis”; normal polarity (500 V/cm)
ZipChip HRN chip (native antibody kit)
ZipChip
ZipChip native Antibody kit
Orbitrap (QE Plus with extended mass Biopharma Option)
mAb: cetuximab
MCE (CZE)
BGE: ZipChip native Antibody kit, 3.8% DMSO; normal polarity (650 V/cm)
ZipChip HRN chip (native antibody kit)
ZipChip
ZipChip native Antibody kit
Orbitrap (QE-EMR)
mAb: NISTmAb
ACE
25 mM AmAc (pH 8.0); reverse polarity (30 kV)
PB-DS-PB
Coaxial SL
IPA/water/25 mM AmAc (pH 8.0) (25:50:25, v/v/v)
Q-TOF (micrOTOF-QII)
Bovine trypsinogen, trypsin, α-chymotrypsin, bovine aprotinin
ACE
BGE: 50 mM AmAc (pH 6.0); normal polarity (20 kV)
PAM
Sheathless porous tip
not applicable
Orbitrap (QE-EMR)
FcRn construct, standard and engineered mAbs, Fc-only constructs
ACE
BGE: 50 mM AmAc (pH 6.8); normal polarity (20 kV), 2 psi forward pressure
PAM
Sheathless porous tip
not applicable
FT-ICR (solariX 15 T)
FcγIIa construct, mAb A, C and E