Native
Capillary Electrophoresis-Mass Spectrometry for Protein analysis:
Current Progress and Perspectives
Ann-Katrin Schwenzer1, Lena Kruse1,
Kevin Jooß2, Christian Neusüß1
1 Department of Chemistry, Aalen University,
Beethovenstraße 1, 73430 Aalen, Germany
2 Department of Chemistry and Molecular Biosciences,
the Chemistry of Life Processes Institute, and the Proteomics Center of
Excellence, Northwestern University, Evanston, Illinois 60208, United
States
Abbreviations: ACE, affinity capillary electrophoresis; ACD,
antibody-drug conjugate; AmAc, ammonium acetate; AmBicarb ammonium
bicarbonate; AmF ammonium formate; BGE background electrolyte; E.
coli, Escherichia coli ; EPO, erythropoietin; ES, electrospray; FcRn;
neonatal Fc receptor; HRN, high resolution native; HSN, high speed
native; HOS higher order structure; iCIEF, imaged CIEF; IM-MS, ion
mobility-mass spectrometry; IFN-γ, interferon-gamma; IPA, isopropanol;
Kd, affinity constant; LIF, light-induced-fluorescence;
LPA, linear polyacrylamide; MCE, microchip electrophoresis; MoCE,
mobility capillary electrophoresis; msACE mobility shift affinity
capillary electrophoresis, nanoSL, nanoflow sheath liquid, nMS native
mass spectrometry, nCIEF, native capillary isoelectric focusing; nCZE,
native capillary zone electrophoresis; nMCE, native microchip
electrophoresis; PB-DS-PB, polybrene-dextran sulfate-polybrene; PVA,
polyvinyl-alcohol; SL, sheath liquid; SMIL, successive multiple ionic
polymer layer; TD, top-down; TDMS top-down mass spectrometry; UV,
ultraviolet.