CZE
|
BGE: 50 mM AmAc (pH 8.3);
normal polarity (30 kV)
|
uncoated
|
Coaxial SL
|
10 mM AmAc (pH 6.8)/ MeOH (50:50, v/v)
|
TOF
(Mariner)
|
Myoglobin
|
|
CZE
|
BGE: 50 mM AmAc (pH 7.4);
reverse (20 kV), 0.7 psi
|
PVA
|
Coaxial SL
|
IPA/water (50:50, v/v) containing 14 mM AmAc
|
QTOF
(micrOTOFQ,
MAXIS H/D)
|
Antithrombin
|
|
CZE |
BGE: 50 mM AmBicarb (pH 7.4); normal polarity (30 kV) |
uncoated |
Coaxial SL, sheathless porous tip |
IPA/water/formic acid (49.5:49.5:1,
v/v/v) |
TOF (6210 LC/MS), QTOF (maXis HD Ultra high resolution) |
Beta2-microglobulin |
|
CZE
|
BGE: 40 mM AmAc (pH 7.5); normal polarity (20 kV), 1-3 psi
|
PAM
|
Sheathless porous tip
|
not applicable
|
Orbitrap
(QE-EMR,
QE Plus)
|
E. Coli ribosomal extract, standard proteins, mAb:
trastuzumab
|
|
SEC-CZE
|
BGE: 50 mM AmAc (pH 6.9);
normal polarity (15 kV), 1 psi
|
LPA
|
NanoSL
|
25 mM AmAc (pH 6.9)
|
Orbitrap
(QE HF)
|
E. Coli
|
|
CZE |
BGE: 40 mM AmAc (pH 7.5);
normal
polarity (20 kV), 3 psi
|
PAM |
Sheathless porous tip |
not applicable |
Orbitrap
(QE Plus)
|
mAb: IgG1 |
|
CZE
|
BGE: 40 mM AmAc (pH 6.0);
reverse polarity (12 kV)
|
PB-DS-PB
|
Coaxial SL
|
IPA/water/acetic acid (50:50:0.1, v/v/v)
|
QTOF
(QTOF 6540)
|
mAb: infliximab
|
|
CZE |
BGE: 25 mM AmAc (pH 6.8);
normal
polarity (30 kV), 0.5 psi
|
LPA |
NanoSL |
10 mM AmAc |
Orbitrap
(modified QE-EMR)
|
Ribosomal
Proteins |
|
CZE
|
BGE: 25 mM AmAc (pH 6.8);
normal polarity (30 kV), 0.725 psi
|
LPA
|
NanoSL
|
10 mM AmAc (pH 6.8)
|
QTOF
(6545XT AdvanceBio LC/QTOF)
|
mAb: SigmaMAb, NISTmAb
|
|
CZE |
BGE: 40 mM AmAc (pH 6.8);
normal
polarity (15 kV), 3 psi
|
PAM |
Sheathless porous tip |
not applicable |
Orbitrap
(QE-EMR, UHMR, and Eclipse)
|
Standard proteins, GroEL |
|
CZE
|
BGE: 40 mM AmAc (pH 6.8);
normal polarity (18 kV), 2.2-15 psi
|
PAM
|
Sheathless porous tip
|
not applicable
|
Orbitrap
(QE-EMR)
|
Synthetic and
endogenous nucleosomes
|
|
MCE
(CZE)
|
BGE: ZipChip native Antibody kit); normal polarity (500 V/cm)
|
ZipChip HRN chip (native antibody kit)
|
ZipChip
|
ZipChip native Antibody kit
|
Orbitrap
(QE Plus with extended mass Biopharma Option)
|
mAb: IgG4 and IgG1
|
|
MCE
(CZE)
|
BGE: ZipChip native Antibody kit, 4% DMSO; normal polarity
(500 V/cm)
|
ZipChip HRN chip (native antibody kit)
|
ZipChip
|
ZipChip native Antibody kit
|
Orbitrap
(QE Plus with extended mass Biopharma Option)
|
mAb: rituximab, trastuzumab and bevacizumab
|
|
MCE
(CZE)
|
BGE: ZipChip native Antibody kit, 4% DMSO; normal polarity
(500 V/cm)
|
ZipChip HRN chip (native antibody kit)
|
ZipChip
|
ZipChip native Antibody kit
|
Orbitrap
(QE)
|
mAb: IgG1
|
|
MCE
(CZE)
|
BGE: ZipChip protocol ”Boosting Sensitivity for Intact Antibody Charge
Variant Analysis”;
normal polarity (500 V/cm)
|
ZipChip HRN chip (native antibody kit)
|
ZipChip
|
ZipChip native Antibody kit
|
Orbitrap
(QE Plus with extended mass Biopharma Option)
|
mAb: cetuximab
|
|
MCE
(CZE)
|
BGE: ZipChip native Antibody kit, 3.8% DMSO; normal polarity
(650 V/cm)
|
ZipChip HRN chip (native antibody kit)
|
ZipChip
|
ZipChip native Antibody kit
|
Orbitrap
(QE-EMR)
|
mAb: NISTmAb
|
|
ACE
|
25 mM AmAc (pH 8.0); reverse polarity (30 kV)
|
PB-DS-PB
|
Coaxial SL
|
IPA/water/25 mM AmAc (pH 8.0) (25:50:25, v/v/v)
|
Q-TOF
(micrOTOF-QII)
|
Bovine trypsinogen, trypsin, α-chymotrypsin, bovine aprotinin
|
|
ACE
|
BGE: 50 mM AmAc (pH 6.0); normal polarity (20 kV)
|
PAM
|
Sheathless porous tip
|
not applicable
|
Orbitrap
(QE-EMR)
|
FcRn construct, standard and engineered mAbs, Fc-only constructs
|
|
ACE
|
BGE: 50 mM AmAc (pH 6.8); normal polarity (20 kV), 2 psi forward
pressure
|
PAM
|
Sheathless porous tip
|
not applicable
|
FT-ICR
(solariX 15 T)
|
FcγIIa construct, mAb A, C and E
|
|