Materials
Label-free 384-well Corning® Epic®plates were obtained from PerkinElmer and 384-well black walled tissue culture treated plates were from Greiner. DMEM + GlutaMAX, penicillin streptomycin, FBS, MEM NEAA, HEPES, Hygromycin B, trypsin, Hanks balanced salt solution (HBSS) and probenecid were from Life Technologies. NaCHO3 was obtained from Lonza, whilst Pluronic, β-alanine, PD123,319 and glucose were obtained from Sigma Aldrich. Thioridazine Hydrochloride was from Tocris, MU-6840 was purchased from BIONET, CyQUANT assay system was from Thermo Fisher and calcium 6 dye was obtained from Molecular Devices. CHO cells expressing human recombinant MRGPRD receptors were from TCG LifeSciences.