Key Results
Concentration-dependent increases in intracellular
Ca2+ and DMR response were observed upon the addition
of MRGPRD agonists which were similar in rank order but with an
approximately log-unit reduction in pEC50 between
Ca2+ responses and DMR, respectively. Inhibition of
Gq-G protein coupling abolished Ca2+responses whereas DMR responses were sensitive to Gi-G
protein inhibition. The putative MRGPRD antagonists thyoradizine
hydrochloride, and MU-6840, produced different patterns of inhibition
between the two assays whereas PD123,319 was ineffective.