Materials
Label-free 384-well Corning® Epic®plates were obtained from PerkinElmer and 384-well black walled tissue
culture treated plates were from Greiner. DMEM + GlutaMAX, penicillin
streptomycin, FBS, MEM NEAA, HEPES, Hygromycin B, trypsin, Hanks
balanced salt solution (HBSS) and probenecid were from Life
Technologies. NaCHO3 was obtained from Lonza, whilst
Pluronic, β-alanine, PD123,319 and glucose were obtained from Sigma
Aldrich. Thioridazine Hydrochloride was from Tocris, MU-6840 was
purchased from BIONET, CyQUANT assay system was from Thermo Fisher and
calcium 6 dye was obtained from Molecular Devices. CHO cells expressing
human recombinant MRGPRD receptors were from TCG LifeSciences.