CHO-MRGPRD cells
CHO cells, either untransfected or stably expressing MRGPRD, were
maintained in Dulbecco’s modified Eagles’ medium (DMEM) nutrient mixture
+ GlutaMAX, supplemented with 25mM HEPES, 10% foetal bovine serum
(FBS), 0.6% penicillin–streptomycin, hygromycin B (500
µg.ml-1), NaCHO3 (2.1
g.l-1) and 1x MEM NEAA. Cells were maintained at 37 °C
and 5% CO2 in their media and were passaged twice a
week using Trypsin/EDTA solution.