CyQUANT® Cell Proliferation Assays
Antagonists were added to 384-well tissue culture treated black walled clear flat-bottom assay plates before the addition of cells, which were plated at 2000 cells per well in DMEM + GlutaMAX, supplemented with 25mM HEPES, 10% FBS, 0.6% penicillin–streptomycin, hygromycin B (500µg.ml-1), NaCHO3(2.1g.l-1) and 1x MEM-NEAA with and without β-alanine. The plates were incubated for either 0, 24, 48 or 72 hours before a solution of PBS with CyQUANT® stain and background suppressor were added prior to incubation for one hour at room temperature in the dark before reading the fluorescence at excitation/emission wavelengths of 480/535nm.