Background and Purpose
Human mas-related genes such as MRGPRD are implicated in the sensation
and transmission of noxious stimuli such as pain and itch and may prove
useful in the management of unmet patient need. To date there have been
few reports of the generation of inhibitors of these receptors. To aid
discovery of new agonists and antagonists we describe the use of a
label-free assay to study the MRGPRD pharmacology and compare it to
other more traditional methods such as intracellular calcium release.