Background and Purpose
Human mas-related genes such as MRGPRD are implicated in the sensation and transmission of noxious stimuli such as pain and itch and may prove useful in the management of unmet patient need. To date there have been few reports of the generation of inhibitors of these receptors. To aid discovery of new agonists and antagonists we describe the use of a label-free assay to study the MRGPRD pharmacology and compare it to other more traditional methods such as intracellular calcium release.