Human mas-related genes such as MRGPRD are members of the seven
transmembrane spanning G protein coupled receptor super family
implicated in the sensation and transmission of noxious stimuli such as
pain and itch, hence intervening with these may prove useful in the
management of unmet patient need. To date there have been few reports of
the generation of inhibitors of these receptors. To provide new tools to
aid the effort in discovering new agonists and antagonists here we
describe the use of a label-free assay to study the pharmacology of
MRGPRD and compare it to other more traditional methods such as
intracellular calcium release.