CHO-MRGPRD cells
CHO cells, either untransfected or stably expressing MRGPRD, were maintained in Dulbecco’s modified Eagles’ medium (DMEM) nutrient mixture + GlutaMAX, supplemented with 25mM HEPES, 10% foetal bovine serum (FBS), 0.6% penicillin–streptomycin, hygromycin B (500 µg.ml-1), NaCHO3 (2.1 g.l-1) and 1x MEM NEAA. Cells were maintained at 37 °C and 5% CO2 in their media and were passaged twice a week using Trypsin/EDTA solution.