CyQUANT® Cell Proliferation Assays
Antagonists were added to 384-well tissue culture treated black walled
clear flat-bottom assay plates before the addition of cells, which were
plated at 2000 cells per well in DMEM + GlutaMAX, supplemented with 25mM
HEPES, 10% FBS, 0.6% penicillin–streptomycin, hygromycin B
(500µg.ml-1), NaCHO3(2.1g.l-1) and 1x MEM-NEAA with and without β-alanine.
The plates were incubated for either 0, 24, 48 or 72 hours before a
solution of PBS with CyQUANT® stain and
background suppressor were added prior to incubation for one hour at
room temperature in the dark before reading the fluorescence at
excitation/emission wavelengths of 480/535nm.