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\begin{document}

\title{Nrf2 attenuates methamphetamine-induced myocardial injury by regulating
oxidative stress and apoptosis in mice}



\author[1]{Hao Yu}%
\author[2]{Yanxia Peng}%
\author[2]{Wenjuan Dong}%
\author[2]{Baoyu Shen}%
\author[2]{Genmeng Yang}%
\author[2]{Qianyun Nie}%
\author[2]{Chunhui Song}%
\author[2]{Binzheng Chen}%
\author[2]{Lixiang Qin}%
\author[2]{Yan Tian}%
\author[2]{Yongna Zhao}%
\author[2]{Lihua Li}%
\author[2]{Shijun Hong}%
\affil[1]{Sichuan University West China Hospital}%
\affil[2]{Kunming Medical University}%


\vspace{-1em}



  
  \date{May 08, 2024}


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\begin{abstract}
Objectives: We aimed to clarify the effects of methamphetamine (MA) on
myocardial injury, oxidative stress, and apoptosis in myocardial cells
and to explore the potential mechanism of nuclear factor-erythroid
factor 2-related factor 2 (Nrf2) in MA-induced oxidative stress and
apoptosis. Methods: An acute mice cardiac toxicity model of MA was
established by intraperitoneal injection of MA (2 mg/kg) for 5 days.
Nrf2 activation (by sulforaphane (SFN)) and Nrf2 gene knockout were
performed to explore the regulatory effects of Nrf2 on cardiac toxicity.
Results: The protein expression levels of Nrf2 and HO-1 were increased,
suggesting that MA activated the Nrf2/HO-1 pathway. In the MA group,
cardiac troponin I (cTnI) protein expression increased. Malondialdehyde
(MDA) content increased, superoxide dismutase (SOD) activity decreased.
Protein expression levels of Caspase-3 and Bax increased, and protein
expression levels of Bcl-2 decreased. These changes were reversed by
activation of Nrf2 but became more pronounced after Nrf2 knockout,
suggesting that the activation and knockout of Nrf2 attenuated and
aggravated MA-induced myocardial injury, oxidative stress and apoptosis
in myocardial cells, respectively. Conclusions: MA administration
induced myocardial injury, oxidative stress, and apoptosis in mice. Nrf2
attenuated MA-induced myocardial injury by regulating oxidative stress
and apoptosis, thus playing a protective role.%
\end{abstract}%



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\verb`supplementary file: original full blots pictures.docx`  available at \url{https://authorea.com/users/591987/articles/627713-nrf2-attenuates-methamphetamine-induced-myocardial-injury-by-regulating-oxidative-stress-and-apoptosis-in-mice}

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