Blood osmolality
Blood osmolality data were collected for all treatments at LER in all experiments (Figure 1A). Blood osmolality was significantly higher (p < 0.05) for all treatments compared with control except 21g/kg and 55g/kg. Blood osmolality was 810 mOsm ± 92.6 at LER for the 85g/kg treatment and 797 mOsm ± 107.7 at LER for the 105g/kg treatment, both significantly higher (p < 0.05) than short-term and long-term FW handling controls. Blood osmolality was 389 mOsm ± 19.1 following 10 weeks at 75g/kg, and 353.9 mOsm ± 6.38 for the handling control. Following 24 hours after acclimation to 75g/kg in this experiment, blood osmolality was 399 ± 19.2. Acute salinity stress following transfer to 32g/kg increased blood osmolality to 655 mOsm ± 20.8 at LER (n=8).