Data Dependent Acquisition (DDA) proteomics
DDA spectra were acquired with an Impact II (Bruker), peak lists
generated with DataAnalysis 4.4 (Bruker), and peptide to spectrum
matches identified with PEAKS suite X plus (Bioinformatics Solutions
Inc., Waterloo, Canada) and X!Tandem Alanine (The GPM) using theO. niloticus proteome database downloaded from NCBI RefSeq on
Feb. 25, 2020, supplemented with known O. mossambicus protein
sequences using previously described parameters [29]. Mass tolerance
limits were set at 10 ppm for precursors and 0.03 Da for fragment ions.
All DDA data and metadata are available at MassIVE (MSV000085745) and
ProteomeXchange (PXD020364).