STRING networks for pejus and pessimus range physiological
states
All treatments 85g/kg and greater represent pessimum range salinities
given the ultimate LER endpoint at these salinities. Network analysis
was run for the 78 proteins shared by short-term and long-term salinity
treatments at 85g/kg and 105g/kg and compared with the networks for the
87 proteins unique to the extended 75g/kg treatment. Networks were
identified with greater than three nodes and classified using
ontological term enrichment. Pejus range regulation consisted of a
network of mostly upregulated proteins with enrichment in amino acid
metabolism and oxidoreductase terms including lactate dehydrogenase
(LDH) and glycogen synthase, a network of up and down-regulation of
structural proteins, especially myosin subunits, and downregulation of
proteasome proteins (Figure 5A). Pessimum range networks contained
specific upregulated mitochondrial proteins, with subunits in the
cytochrome b-c1 complex as central members, and downregulation of
extracellular matrix proteins (ECM) with several collagen subunits
(Figure 5B).