Sample processing for proteomics
Seven treatments were chosen to capture states near the critical
salinity threshold. Eight samples from each treatment were taken from
the short-term 85g/kg and 105g/kg treatments and corresponding FW
control, and from the long-term 75g/kg treatment and FW control. Six
replicates were sampled from the acclimation to LER at 85g/kg and
105g/kg. Extraction, in-solution trypsin digestion of proteins, and
peptide separation using a nanoAcquity UPLC system equipped with
Symmetry trap and 250 mm x 1.7µm particle size BEH C18 columns were
performed as previously reported [29].