Data Dependent Acquisition (DDA) proteomics
DDA spectra were acquired with an Impact II (Bruker), peak lists generated with DataAnalysis 4.4 (Bruker), and peptide to spectrum matches identified with PEAKS suite X plus (Bioinformatics Solutions Inc., Waterloo, Canada) and X!Tandem Alanine (The GPM) using theO. niloticus proteome database downloaded from NCBI RefSeq on Feb. 25, 2020, supplemented with known O. mossambicus protein sequences using previously described parameters [29]. Mass tolerance limits were set at 10 ppm for precursors and 0.03 Da for fragment ions. All DDA data and metadata are available at MassIVE (MSV000085745) and ProteomeXchange (PXD020364).