Figure 2 - Representative 1H-MRS chemical shift imaging brain slice orientation on 3 planes (A – axial, B – coronal, C – sagittal) and LCModel spectra of VOIs which included: (D & G) bilateral dorsolateral prefrontal cortices (DLPFC), (E & H) bilateral frontal white matter (FWM) and (F & I) bilateral anterior cingulate cortices (ACC).
Bloods were collected via venepuncture into serum tubes that were kept a room temperature for 30 minutes to allow for clotting. The tubes were centrifuged, serum samples were collected into cryovials and immediately stored at -80 °C. The immune markers IL-1β, IL-8, IL-10, TNF-α and IFN-γ, were analyzed with a Milliplex® Luminex human cytokine magnetic bead panel (HSTCMAG28SK07; Merck) according to the manufacturer’s instructions. Plates were assayed on a Luminex system (Bio-Plex 200 System: Bio-Rad). All samples were blinded and assayed in duplicate. The intra-assay coefficients of variation for each cytokine marker were < 14%.
For group differences, parametric data were analyzed with one-way analysis of variance and non-parametric data analyzed with Kruskal Wallis and Chi Square tests, with post-hoc testing using Fisher’s Least Significant Difference for parametric data. Associations were determined using Spearman’s rank-order coefficient. Significant associations were followed by correcting for multiple comparisons.