Figure 2 - Representative 1H-MRS chemical shift imaging brain
slice orientation on 3 planes (A – axial, B – coronal, C – sagittal)
and LCModel spectra of VOIs which included: (D & G) bilateral
dorsolateral prefrontal cortices (DLPFC), (E & H) bilateral frontal
white matter (FWM) and (F & I) bilateral anterior cingulate cortices
(ACC).
Bloods were collected via venepuncture into serum tubes that were kept a
room temperature for 30 minutes to allow for clotting. The tubes were
centrifuged, serum samples were collected into cryovials and immediately
stored at -80 °C. The immune markers IL-1β, IL-8, IL-10, TNF-α and
IFN-γ, were analyzed with a Milliplex® Luminex human cytokine magnetic
bead panel (HSTCMAG28SK07; Merck) according to the manufacturer’s
instructions. Plates were assayed on a Luminex system (Bio-Plex 200
System: Bio-Rad). All samples were blinded and assayed in duplicate. The
intra-assay coefficients of variation for each cytokine marker were
< 14%.
For group differences, parametric data were analyzed with one-way
analysis of variance and non-parametric data analyzed with Kruskal
Wallis and Chi Square tests, with post-hoc testing using Fisher’s Least
Significant Difference for parametric data. Associations were determined
using Spearman’s rank-order coefficient. Significant associations were
followed by correcting for multiple comparisons.