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\begin{document}

\title{Integrative systems biology of wheat susceptibility to \emph{Fusarium
graminearum} identifies gene regulatory networks and conserved master
regulators}



\author[1]{Florian Rocher}%
\author[1]{Samir Dou}%
\author[1]{Géraldine Philippe}%
\author[2]{Marie-Laure Martin}%
\author[3]{Philippe Label}%
\author[1]{Thierry Langin}%
\author[1]{Ludovic Bonhomme}%
\affil[1]{Genetique Diversite Ecophysiologie des Cereales}%
\affil[2]{Universite Paris-Saclay Departement de Biologie}%
\affil[3]{Physique et Physiologie Integratives de l'Arbre en Environnement Fluctuant}%


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  \date{June 03, 2024}


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\begin{abstract}
\textbf{Background:~}Plant diseases are driven by an intricate set of
defense mechanisms counterbalanced by the expression of host
susceptibility factors promoted through the action of pathogen
effectors. In spite of their central role in the establishment of the
pathology, the primary components of plant susceptibility are still
poorly understood and challenging to trace especially in plant-fungal
interactions such as in Fusarium head blight (FHB) of bread wheat.
Designing a system-level transcriptomics approach, we leveraged the
analysis of wheat responses from a susceptible cultivar facing
\emph{Fusarium graminearum} strains of different aggressiveness and
examined their constancy in four other wheat cultivars also developing
FHB.

\textbf{Results:} In this study, we describe unexpected differential
expression of a conserved set of transcription factors and an original
subset of master regulators were evidenced using a regulation network
approach. The dual-integration with the expression data of pathogen
effector genes combined with database mining, demonstrated robust
connections with the plant molecular regulators and identified relevant
candidate genes involved in plant susceptibility, mostly able to
suppress plant defense mechanisms. Furthermore, taking advantage of
wheat cultivars of contrasting susceptibility levels, a refined list of
142 conserved susceptibility gene candidates were proposed to be
necessary host's determinants for the establishment of a compatible
interaction.

\textbf{Conclusions:} Our findings emphasized major FHB determinants
potentially controlling a set of conserved responses associated with
susceptibility in bread wheat. They provide new clues for improving FHB
control in wheat and also could conceivably leverage further original
researches dealing with a broader spectrum of plant pathogens.%
\end{abstract}%



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\textbf{Hosted file}

\verb`WheatFHBresponses_Rocher_et_al_vf.docx`  available at \url{https://authorea.com/users/589267/articles/626175-integrative-systems-biology-of-wheat-susceptibility-to-fusarium-graminearum-identifies-gene-regulatory-networks-and-conserved-master-regulators}



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