Comet assay
As described in our recent publication (Dong et al., 2022), after
carefully filling 0.5% LMP (Low-melting-point) agarose flowing at
liquid state above the normal melting point agarose, oocytes were
transferred quickly into the LMP layer and then placed at 4°C for
LMP liquid solidification, followed by soaking in lysing solution at 4°C
for 1h, and washing with distilled water for 10 min, then transfer into
alkaline buffer for 20 min on ice before electrophoresis at 25V for 20
min. Finally, the oocytes were stained with EB (20μg/ml) for 15 min and
then examined with a fluorescence microscope (Leica, Germany). CASP
software was used to analyze the comet tail length and comet tail DNA.