Evaluation of Striga resistance in pot experiments
The resistance response of 13 SAP lgs1 sorghum lines alongside controls was further studied in pots under greenhouse conditions as described by Jamil et al . (2022b). A mixture of soil (Stender), sand and rock at a 3:1:1 ratio was prepared. About 1 L of uninfected soil was added to the bottom of each 3 L plastic pot. Then, about 120 mg (~70000) Striga seeds were mixed thoroughly with 6 L soil mixture which was added equally in six pots (1 L per pot) containing uninfected soil. The pots were irrigated and Strigaseeds allowed to condition for 10 days. Following conditioning, one-week-old sorghum seedlings (one per pot) were planted. The number of emerged Striga seedlings and plant height were scored in each pot 10 weeks after sorghum planting.