Molecular characteristics of OsAP79
It has been shown that OsAP79 plays a positive role in rice BPH
resistance. Thus, we explored the potential roles of its molecular
characteristics. To test whether OsAP79 was responsive to BPH
infestation, we quantified its expression at different time points after
BPH treatment. The expression of OsAP79 was induced and
continuously increased when NIP was treated with BPH during a period of
96 h, and it was significantly high at the time points of 72 h and 96 h
(Figure 3a).
We then checked its expression in different organs of NIP using qRT-PCR.
The results showed that OsAP79 was expressed in all organs at the
seedling and adult stages. However, the expression level was
significantly higher in the roots than in the other organs, especially
at the adult stage (Figure 3b).
To further dissect the expression pattern of OsAP79 at the
subcellular level, we expressed OsAP79-GFP in rice protoplasts. We
speculated that OsAP79 was expressed in the endoplasmic reticulum (ER)
(Figure S6), and then co-expressed the fusion protein with an
endoplasmic reticulum-red fluorescent protein (ER-RFP) marker in rice
protoplasts. As a result, the fluorescence signals of the two fusion
proteins are highly overlapped (Figure 3c), suggesting that OsAP79 was
predominantly localized in the ER.