Phylogenetic and sequence analyses
To develop a phylogenetic tree of OsAP79 , BLASTP searches against all annotated proteins in the TIGR pseudomolecules (version 5), with OsAP79 as the query, were compared and defined into subgroups using the hidden Markov model profile. The neighbor-joining tree was developed using the p-distance substitution model in MEGA 4, and the bootstrap support values were also calculated. OsAP79 alleles of rice varieties/lines RBPH16, PTB33, RBPH327, ARC5984, 9311, NIP, ZH11, and BaiMao were amplified and sequenced. Sequence alignments ofOsAP79 homologs were performed using DNAMAN.