Agronomic trait measurement
To detect agronomic traits, five rows were cultivated at a density of 20 × 30 cm (20 cm spacing between plants and 30 cm spacing between rows) in 2020; each row, included ten plants of each line, OE, knockout, and NIP. Three repeats were randomly arranged in the rice field. Twenty plants except for the ones outside were then chosen, and their agronomic traits including PH, PL, TN, SR, and GWT were surveyed. Field management comprised normal agronomic practices.