Subcellular localization of OsAP79
To construct a subcellular localization test, the full-length cDNA ofOsAP79 was cloned into pCAMBIA2300-35S. The GFP-OsAP79 construct was then transformed alone or co-transformed with the ER-RFP marker into rice protoplasts using a previously described method (Zhang et al ., 2011). Finally, the rice protoplasts were observed under a confocal laser scanning microscope (Leica TCS SP5, Germany) after incubating for 24 h.