BLB resistance test
To perform the BLB resistance test, a virulent Xoo strain,
GX1070, was collected from rice plants from Guangxi Province in China,
and cultured on nutrient agar (NA) medium at 28 °C for 4 days. The
bacteria were then collected and dissolved in ddH2O to
an optical density of OD600 = 1.0. The 30-day-old plants were then
inoculated with bacteria using the leaf-clipping method, and the lesion
length of each leaf was measured after 3 weeks of inoculation. Four
plants were used for each line and the test was repeated twice.