Phylogenetic and sequence analyses
To develop a phylogenetic tree of OsAP79 , BLASTP searches against
all annotated proteins in the TIGR pseudomolecules (version 5), with
OsAP79 as the query, were compared and defined into subgroups using the
hidden Markov model profile. The neighbor-joining tree was developed
using the p-distance substitution model in MEGA 4, and the bootstrap
support values were also calculated. OsAP79 alleles of rice
varieties/lines RBPH16, PTB33, RBPH327, ARC5984, 9311, NIP, ZH11, and
BaiMao were amplified and sequenced. Sequence alignments ofOsAP79 homologs were performed using DNAMAN.