Figure Legends
Figure 1. (a) Relative expression of Os10g39300 after
BPH infestation. Asterisks represent significant differences based on
one-way ANOVA and Tukey test (*, P < 0.05; **, P< 0.01). (b) Phylogenetic analysis of the conserved ASP domain
from rice OsAPs and RBPH16. The phylogenetic tree was constructed by the
neighbor-joining method in MEGA 4.0 software (p-distance model; 1000
bootstraps). Scale bar represents 0.2 amino acid substitution per site.
Figure 2. BPH resistance evaluation of lines overexpression
(OE) and knockout (CR) of OsAP79 . BPH-resistance assay of lines
OE, CR and Nipponbare (NIP) at seedling (a) or adult stage (c). Scale
bar, 5 cm. Survival rates of seedling (b) and adult (d) detected with
the homozygous T2 lines of OE and CR of OsAP79 .
BPH weight gain (e) and Honeydew excretion (f) by newly emerged female
adult BPHs fed on the lines of OE, CR and NIP for 48 h. Asterisks
represent significant difference based on one-way ANOVA and Tukey test
(*, P < 0.05; **, P < 0.01).
Figure 3. Molecular characteristics of OsAP79 . (a)
Relative expression of gene OsAP79 in Nipponbare (NIP) after BPH
infestation. (b) Relative expression of OsAP79 in different
organs of NIP. Asterisks in (a) and (b) represent significant
differences based on one-way ANOVA and Tukey test (*, P< 0.05; **, P < 0.01. (c) Rice protoplasts
were co-transfected with endoplasmic reticulum-red fluorescent protein
(ER-RFP) and OsAP79-GFP. The transfected cells were examined under a
confocal microscope. The images were then processed using ZEISS ZEN
confocal microscope software. Overlapping fluorescence signals show that
OsAP79 localizes to the ER. Bright-field images are shown on the right.
Scale bars, 10 μm.
Figure 4. Root length detection of lines overexpression (OE),
knockout (CR) and Nipponbare (NIP). (a) Five days after germination,
plants were grown hydroponically for 30 days under normal nutrient
solution conditions. Scale bars: 1 cm. (b) The root growth phenotype of
plants growing in the soil. Adults grown on were separated carefully
from the soil transferred. Scale bars: 10 cm. Main root length was
measured at seedlings (c) and adults (d). (e) Relative root length rate
in 5d and 10d (1μM IAA treated versus untreated in each genotype,
respectively). IAA (f) and SA (g) content of 20-day-old normal grown
seedlings. Asterisks in (c, d, e, f and g) represent significant
differences based on one-way ANOVA and Tukey test (*, P< 0.05; **, P < 0.01).
Figure 5. Rice sclerenchyma layer thickness detection.
Representative images of making free hand sections to detect the
sclerenchyma layer thickness in stem cross-sections of lines
overexpression (OE), knockout (CR) and Nipponbare (NIP). (a) seedling;
(b) adult. Scale bars, 1000μm. The thickness of sclerenchyma layer in
stem transverse sections at seedling (c) and adult stage (d) was
measured. Asterisks in (c,d) represent significant differences based on
one-way ANOVA and Tukey test (*, P < 0.05; **, P< 0.01).
Figure 6. Metabolites were mapped onto network of metabolic
pathways. The contents of metabolites after BPH feeding at different
time were represented by Graphics with different Position. Each graph
represents the fold change considering the time 0h as a control; columns
represent the two evaluated times for each metabolite. The first row
corresponds to the Nipponbare (NIP), whereas the second row corresponds
to the overexpression (OE) (a) or knockout (CR) (b). Red symbols denote
significant increases (P < 0.05); black symbols denote
no significant changes (P > 0.05); green symbols
denote significant decreases (P < 0.05). The upstream
and downstream relationship of these metabolites was indicated by
direction of arrow. Solid lines denote direct correlations among each of
metabolites. Dotted lines denote that relative long pathway
transformation occurred among each of metabolites.
Table 1 Agronomic trait detection of Nipponbare, overexpression
(OE) and knockout (CR) lines.