Detection of OsAP79 expression with BPH treatment
To explore the function of AP genes in BPH resistance in rice, eight
genes were detected in the transcriptome of resistant and susceptible
rice lines after BPH infection (Table S1). Among these, compared with
that of the susceptible line 9311, the expression of Os10g39300was continuously increased in two different genotypic lines after BPH
infestation, whereas a large fold increase was detected in the resistant
line RBPH16. However, this phenomenon was not observed for the
expression of the other seven AP genes. Furthermore, quantitative
real-time PCR (qRT-PCR) analysis confirmed the same expression model forOs10g39300 when the resistant and susceptible lines were treated
with BPH insects for a period of 96 h (Figure 1a). Therefore, we
inferred that Os10g39300 was most likely associated with BPH
resistance in rice; thus, we selected it to explore its function in
further studies.