Transgenic plant assay
To detect the positive transgenic plants of OsAP79 , knockout
plants were identified through Sanger sequencing of the PCR products
spanning the target regions in the T0 generation. For
the OE plants, the copy number of the transgene was estimated by
comparing it with the standard single copy sample in the
T0 generation, and verified their expression in the
T1 generation using qRT-PCR (Yang et al ., 2004).
All the lines selected for further studies were surveyed using the BPH
resistance test.