Subcellular localization of OsAP79
To construct a subcellular localization test, the full-length cDNA ofOsAP79 was cloned into pCAMBIA2300-35S. The GFP-OsAP79 construct
was then transformed alone or co-transformed with the ER-RFP marker into
rice protoplasts using a previously described method (Zhang et
al ., 2011). Finally, the rice protoplasts were observed under a
confocal laser scanning microscope (Leica TCS SP5, Germany) after
incubating for 24 h.