The location and origin of Neoproterozoic-Cambrian sutures provide keys to understand the formation and evolution of the supercontinent Gondwana. The Larsemann Hills is located near a major Neoproterozoic-Cambrian suture zone in the Prydz Belt, but has not been examined locally by comprehensive geophysical studies. In this study, we analyzed data collected from a 1-D joint seismic-MT array deployed during the 36th Chinese National Antarctic Research Expedition. We found that a sharp Moho discontinuity offset of 6-8 km shows up in the stacked image of teleseismic P-wave receiver function analysis; coinciding with the abrupt Moho offset, a near-vertical channel with (1) low resistivity extending to the uppermost mantle depths, and (2) a high crustal Poisson’s ratio in the crust is identified. These findings provide evidence for the determination of the location and collisional nature of the Prydz belt or a portion of it.