Modified Technique in Sample Preparation for Light Microscopy with Oil
Immersion Objective Lens Imaging
Abstract
Background: There is a growing need for contemporary
application of microscopic techniques in laboratory practice. Moreover,
the oil immersion microscopy is rarely done because of its intricacy. As
such concerned scientists need to be abreast of the step-by-step
procedures for preparing a biological specimen on the whole as well as
method for increasing the resolving power of a microscope. Aim:
To prepare slides from various organ systems with photomicrographs of
different resolutions. Materials and Methods: Tissue samples
were sourced from laboratory Wistar rats ( Rattus norvegicus).
Other research items included histopathology lab reagents as well as
equipment including embedding mould, water bath, oven and rotary
microtome. Standard method of paraffin embedding was used with some
modification and sections stained with hematoxylin (Harris) and eosin.
Images were prepared using a photomicroscope connected to a computer
interface. Results: 500 pieces of microscope slides
encompassing 20 different organ/tissue samples, with each mounted on 25
glass slides, while the photomicrographs taken at low (x100), high
(x400) and higher (x1000) power magnifications were produced from the
various specimens and saved on a disk. Conclusion: The outcome
demonstrates the adequacy of the histological protocol used to guide
researchers in related fields.