Sequencing and phylogenetic analysis
Amplified DNA fragments of 1,210bp were used forthe dideoxynucleotide
sequencing reactions. PCR-amplified products were analyzed on an ABI
Prism 3700 automated DNA sequencer (Applied Biosystems, Foster City,
California). Low quality sequences were trimmed with Chromas version
2.6.2 for windows licensed by Technelysium Pty Ltd 1998-2016
(www.technelysium.com.au). The sequences were combined with those
currently available from GenBank (as shown in Table 2). Pairwise and
multiple genomic alignments were done with Clustal
W13 alignment programs. The evolutionary
history was inferred by using the Maximum Likelihood (ML) method and
Kimura 2-parameter model14 . The percentage of
trees in which the associated taxa clustered together is shown next to
the branches. Initial tree(s) for the heuristic search were obtained
automatically by applying ML algorithm with a GTR+G model, and 1000
bootstrap resampling. The tree is drawn to scale, with branch lengths in
the same units as those of the evolutionary distances used to infer the
phylogenetic tree. This analysis involved 55 nucleotide sequences with
556 positions in the final dataset. Evolutionary analyses were conducted
in iqtree15 . Sequences were submitted to the
GenBank and accession numbers MW916908-MW916918 were assigned for 11
isolates in this present study. Information regarding the study
sequences can be accessed in supplementary table 1.